Issue 121: enzyme combinations (N and C terminal)

issues
Status:closed
Assigned To:Guest
Type:Todo
Area:Skyline
Priority:3
Milestone:1.4
Opened:2011-12-05 by Birgit Schilling
Changed:2013-08-09 by Brendan MacLean
Resolved:2013-08-09 by Brendan MacLean
Resolution:Fixed
Closed:2013-08-09 by Brendan MacLean
2011-12-05 Birgit Schilling
Title»enzyme combinations (N and C terminal)
Assigned To»Brendan MacLean
Type»Todo
Area»Skyline
Priority»3
Milestone»1.2
Hi Brendan,
 
we have a project where we are using enzyme combinations.
 
It is easy to define enzyme combinations if say both enzymes are C-terminal or both enzymes are N-terminal with their cleavage specificity. However, here we have a combination where one is N-terminal and one is C-terminal.
 
How do we define that in Skyline ?
 
See screenshot, that is what we would like to combine (and then we have more examples just like that).
thanks very much for any thoughts Brendan!

in Skyline can we allow combinations of enzymes, or custom enzymes with AAs for both directions.
 
Birgit
 
 

2013-08-09 Brendan MacLean
resolve as Fixed
Statusopen»resolved
Assigned ToBrendan MacLean»Birgit Schilling
Milestone1.2»1.4
This was fixed for v1.4. It is now possible to define an enzyme (protease) that cleaves both C-terminal to some AA residues and N-terminal to others.

2013-08-09 Brendan MacLean
close
Statusresolved»closed
Assigned ToBirgit Schilling»Guest