| Title | | » | ModificationMatcher over guesses with Acetyl modifications |
| Assigned To | | » | Nick Shulman |
| Type | | » | Defect |
| Area | | » | Skyline |
| Priority | | » | 2 |
| Milestone | | » | 23.2 |
Download brendanx@nexus:data\MacCoss\MaxQuant\Label Free\Label-free.sky.zip
Open the .sky.zip file in Skyline.
View > Spectral Libraries.
Bug: Skyline asks to use 3 Acetyl modifications to explain the modifications in the library:
Acetyl (N-term)
Acetyl (S)
Acetyl (T)
If you go to Peptide Settings - Modifications and add just Acetyl (N-term) and then View > Spectral Libraries again, Skyline drops all suggestions for adding Acetyl modifications, because Acetyl (N-term) explains everything in the library.
Maybe worth noting only Acetyl (N-term) and Acetyl (K) show up in the common modifications, while 6 other amino acid-specific Acetyl modifications only appear when you show all modifications.
We should make ModificationMatcher guess better the minimum set of modifications to explain what it sees in a set of modified sequences.