Title | | » | No import due to overlapping chromatogram start times? |
Assigned To | Guest | » | Brendan MacLean |
Notify | | » | blaine roberts |
Type | | » | Defect |
Area | | » | Skyline |
Priority | | » | 3 |
Milestone | | » | 4.3 |
Trying to import ion mobility DIA data with a peptide list generated from an error tolerant peptide search. I suspect the error tolerant peptides are causing the issue. See error text below. I removed the peptides listed below and tired again but another peptide becomes the problem. If I generate the spectral library without the ET results then the import proceeds as normal.
At 12:47 PM:
Failed importing results file 'E:\MassHunter\Data\Blaine\24.01.19.Harvey_gen_Pro\DIA-30ms-IMS-40ug_BrainRef.d'.
Unable to process chromatograms for the molecule 'EGPGEAIVR' because one chromatogram ends at time '14.4538164138794' and the other ends at time '14.4933662414551'.
Inner exceptions:
Exception type: System.InvalidOperationException
Error message: Unable to process chromatograms for the molecule 'EGPGEAIVR' because one chromatogram ends at time '14.4538164138794' and the other ends at time '14.4933662414551'.
Unable to process chromatograms for the molecule 'EGPGEAIVR' because one chromatogram ends at time '14.4538164138794' and the other ends at time '14.4933662414551'.
at pwiz.Skyline.Model.Results.PeptideChromDataSets.AddDataSet(ChromDataSet chromDataSet) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\PeptideChromData.cs:line 838
at pwiz.Skyline.Model.Results.PeptideChromDataSets.Add(PeptideDocNode nodePep, ChromDataSet chromDataSet) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\PeptideChromData.cs:line 794
at pwiz.Skyline.Model.Results.ChromCacheBuilder.AddChromDataSet(Boolean isProcessedScans, ChromDataSet chromDataSet, PeptidePrecursorMz peptidePrecursorMz, IDictionary`2 dictPeptideChromData, ICollection`1 listChromData, ChromFileInfo fileInfo) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 680
at pwiz.Skyline.Model.Results.ChromCacheBuilder.CalcPeptideChromDataSets(ChromDataProvider provider, List`1 listMzPrecursors, HashSet`1 setInternalStandards) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 520
at pwiz.Skyline.Model.Results.ChromCacheBuilder.Read(ChromDataProvider provider) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 393
at pwiz.Skyline.Model.Results.ChromCacheBuilder.BuildCache() in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 263