<?xml version="1.0" encoding="utf-8"?>
<srm_settings format_version="23.1" software_version="Skyline (64-bit) 23.1.0.455 (599e74ad2)">
  <settings_summary name="Default">
    <peptide_settings>
      <enzyme name="Trypsin-GluC" cut="DEKR" no_cut="P" sense="C" />
      <digest_settings max_missed_cleavages="2" />
      <background_proteome name="HP" database_path="H:\DATA_VC\Real samples\test1\result.protdb_1.protdb" />
      <peptide_prediction use_measured_rts="false" measured_rt_window="2" />
      <peptide_filter start="25" min_length="5" max_length="25" auto_select="true" unique_by="protein">
        <peptide_exclusions />
      </peptide_filter>
      <peptide_libraries pick="library" />
      <peptide_modifications max_variable_mods="4" max_neutral_losses="1">
        <static_modifications>
          <static_modification name="Mod_1400_8" aminoacid="N" terminus="C" formula="C124H204N8O90" />
          <static_modification name="Mod_1352_2" aminoacid="N" terminus="C" formula="C119H197N7O86" />
          <static_modification name="Mod_1014_4" aminoacid="N" terminus="C" formula="C119H197N7O86" />
          <static_modification name="Mod_1327_5" aminoacid="N" terminus="C" formula="C115H188N8O84" />
          <static_modification name="Mod_1230_4" aminoacid="N" terminus="C" formula="C104H171N7O76" />
          <static_modification name="Mod_923_1" aminoacid="N" terminus="C" formula="C104H171N7O76" />
          <static_modification name="Mod_1133_4" aminoacid="N" terminus="C" formula="C93H154N6O68" />
          <static_modification name="Mod_1108_7" aminoacid="N" terminus="C" formula="C90H148N6O66" />
          <static_modification name="Mod_1011_7" aminoacid="N" terminus="C" formula="C79H131N5O58" />
          <static_modification name="Carbamidomethyl (C)" aminoacid="C" formula="H3C2NO" unimod_id="4" short_name="CAM" />
          <static_modification name="Mod_2350_8" aminoacid="N" variable="true" formula="C90H146N6O65" />
          <static_modification name="Mod_2424_8" aminoacid="N" variable="true" formula="C93H152N6O67">
            <potential_loss formula="C93H152N6O67" massdiff_monoisotopic="2424.86713" massdiff_average="2426.21593" charge="4" />
            <potential_loss formula="C93H152N6O67" massdiff_monoisotopic="2424.86713" massdiff_average="2426.21593" charge="3" />
          </static_modification>
          <static_modification name="Mod_2715_9" aminoacid="N" variable="true" formula="C104H169N7O75" />
          <static_modification name="Mod_2862_0" aminoacid="N" variable="true" formula="C110H179N7O79" />
          <static_modification name="Mod_3154_1" aminoacid="N" variable="true" formula="C122H199N7O87" />
          <static_modification name="Mod_3373_2_N8" aminoacid="N" variable="true" formula="C130H212N8O93" />
          <static_modification name="Mod_3372_2_N9" aminoacid="N" variable="true" formula="C129H209N9O93" />
          <static_modification name="Mod_3664_3" aminoacid="N" variable="true" formula="C141H229N9O101" />
          <static_modification name="Mod_3809_3" aminoacid="N" variable="true" formula="C146H236N10O105" />
          <static_modification name="Mod_3008_1" aminoacid="N" variable="true" formula="C116H189N7O83" />
          <static_modification name="Mod_1913_7" aminoacid="N" variable="true" formula="C73H119N5O53" />
          <static_modification name="Mod_2939_1" aminoacid="N" variable="true" formula="C112H182N8O81" />
          <static_modification name="Mod_2221_8" aminoacid="N" variable="true" formula="C85H139N5O62" />
          <static_modification name="Mod_2512_9" aminoacid="N" variable="true" formula="C96H156N6O70" />
          <static_modification name="Mod_2204_8" aminoacid="N" variable="true" formula="C84H136N6O61" />
        </static_modifications>
        <heavy_modifications />
      </peptide_modifications>
      <quantification weighting="none" fit="none" normalization="tic" ms_level="2" />
    </peptide_settings>
    <transition_settings>
      <transition_prediction precursor_mass_type="Monoisotopic" fragment_mass_type="Monoisotopic" optimize_by="None" />
      <transition_filter precursor_charges="2,3,4" product_charges="1,2,3,4" precursor_adducts="[M+H]" product_adducts="[M+]" fragment_types="y,b,p" small_molecule_fragment_types="f,p" fragment_range_first="m/z &gt; precursor" fragment_range_last="6 ions" precursor_mz_window="0" auto_select="true">
        <measured_ion name="C-terminal to Glu or Asp" cut="ED" sense="C" min_length="3" />
      </transition_filter>
      <transition_libraries ion_match_tolerance="0.02" min_ion_count="0" ion_count="3" pick_from="none" />
      <transition_integration>
        <synchronize_integration group_by="" all="true" />
      </transition_integration>
      <transition_instrument min_mz="50" max_mz="1800" mz_match_tolerance="0.55" />
      <transition_full_scan acquisition_method="PRM" product_mass_analyzer="centroided" product_res="10" precursor_isotopes="Count" precursor_isotope_filter="3" precursor_mass_analyzer="centroided" precursor_res="10" retention_time_filter_type="ms2_ids" retention_time_filter_length="5">
        <isotope_enrichments name="Default">
          <atom_percent_enrichment symbol="H'">0.98</atom_percent_enrichment>
          <atom_percent_enrichment symbol="C'">0.995</atom_percent_enrichment>
          <atom_percent_enrichment symbol="C&quot;">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="N'">0.995</atom_percent_enrichment>
          <atom_percent_enrichment symbol="O&quot;">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="O'">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="Cl'">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="Br'">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="P'">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="S&quot;">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="S'">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="H&quot;">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="Cu'">0.99</atom_percent_enrichment>
        </isotope_enrichments>
      </transition_full_scan>
    </transition_settings>
    <data_settings document_guid="a887bacc-259f-44ce-ab51-e316c4af3455" />
    <measured_results time_normal_area="true">
      <replicate name="C2474">
        <sample_file id="C2474_f0" file_path="H:\DATA_VC\Real samples\03292024\C2474.raw" sample_name="C2474" sample_id="C2474" instrument_serial_number="FSN20322" acquired_time="2024-03-29T10:19:53" modified_time="2024-04-01T09:01:05" import_time="2024-06-27T18:26:54.8072629Z" ion_mobility_type="compensation_V">
          <instrument_info_list>
            <instrument_info>
              <model>Orbitrap Fusion Lumos</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
            <instrument_info>
              <model>Orbitrap Fusion Lumos</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/radial ejection linear ion trap</analyzer>
              <detector>electron multiplier</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
      <replicate name="H2434">
        <sample_file id="H2434_f0" file_path="H:\DATA_VC\Real samples\03292024\H2434.raw" sample_name="H2434" sample_id="H2434" instrument_serial_number="FSN20322" acquired_time="2024-03-29T11:50:56" modified_time="2024-04-01T09:01:20" import_time="2024-06-27T18:26:54.4932447Z" ion_mobility_type="compensation_V">
          <instrument_info_list>
            <instrument_info>
              <model>Orbitrap Fusion Lumos</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/orbitrap</analyzer>
              <detector>inductive detector</detector>
            </instrument_info>
            <instrument_info>
              <model>Orbitrap Fusion Lumos</model>
              <ionsource>nanoelectrospray</ionsource>
              <analyzer>quadrupole/radial ejection linear ion trap</analyzer>
              <detector>electron multiplier</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
    </measured_results>
  </settings_summary>
</srm_settings>