                  INSTRUMENT CONTROL PARAMETERS:    Agilent 5977B
                  -----------------------------------------------

   D:\MassHunter\GCMS\1\methods\Fatty_Acids_160322-20 min.M
      Thu Apr 13 18:20:07 2023

Control Information
------- -----------

Sample Inlet             : GC
Injection Source         : GC ALS
Injection Location:  Rear
Mass Spectrometer        : Enabled


 No Sample Prep method has been assigned to this method.


GC
GC Summary
Run Time                                     20 min
Post Run Time                                0 min

Oven
Equilibration Time                           0.25 min
Max Temperature                              300 °C
Maximum Temperature Override                 Disabled
Slow Fan                                     Disabled
Temperature
Setpoint                                     On
(Initial)                                    40 °C
Hold Time                                    5 min
Post Run                                     70 °C
Program
#1 Rate                                      10 °C/min
#1 Value                                     190 °C
#1 Hold Time                                 0 min



ALS
ALS Errors                                   Pause for user interaction
Back Injector
Syringe Size                                 10 μL
Injection Volume                             1 μL
Solvent A Washes (PreInj)                    1
Solvent A Washes (PostInj)                   1
Solvent A Volume                             4 μL
Solvent B Washes (PreInj)                    0
Solvent B Washes (PostInj)                   0
Solvent B Volume                             8 μL
Sample Washes                                1
Sample Wash Volume                           3 μL
Sample Pumps                                 6
Dwell Time (PreInj)                          0 min
Dwell Time (PostInj)                         0 min
Solvent Wash Draw Speed                      300 μL/min
Solvent Wash Dispense Speed                  3000 μL/min
Sample Wash Draw Speed                       300 μL/min
Sample Wash Dispense Speed                   3000 μL/min
Injection Dispense Speed                     6000 μL/min
Viscosity Delay                              0 sec
Sample Depth                                 Disabled
Injection Type                               Standard
L1 Airgap                                    0.2 μL
Solvent Wash Mode                            A, B

Sample Overlap
Mode                                         Sample overlap is not enabled


Front SS Inlet N2
                                             ***Excluded from Affecting GC's Readiness State***
Mode                                         Sleep
Heater                                       Off
Pressure                                     On    3.3447 psi
Total Flow                                   On    103.5 mL/min
Septum Purge Flow                            On    3 mL/min
Gas Saver                                    On    15 After 2 min mL/min

Back SS Inlet He
Mode                                         Split
Heater                                       On    260 °C
Pressure                                     On    17.342 psi
Total Flow                                   On    54 mL/min
Septum Purge Flow                            On    3 mL/min
Gas Saver                                    Off
Split Ratio                                  50 :1
Split Flow                                   50 mL/min
Liner                                        Agilent 5190-2295: 870 μL (Split, taper, wool, low pressure drop)

Thermal Aux 2 (MSD Transfer Line)
Temperature
Setpoint                                     On
(Initial)                                    280 °C
Post Run                                     0 °C


Column
Column Outlet Pressure                       0 psi
Column #1
Column Information                           Agilent CP9016
VF-5ms                                       
Temperature Range                            -59 °C—449 °C (449 °C)
Dimensions                                   65 m x 250 μm x 0.25 μm
Column lock                                  Locked
In                                           Back SS Inlet He
Out                                          MSD 
(Initial)                                    40 °C
Pressure                                     17.342 psi
Flow                                         1 mL/min
Average Velocity                             24.635 cm/sec
Holdup Time                                  4.3975 min
Flow
Setpoint                                     On
(Initial)                                    1 mL/min
Post Run                                     1 mL/min


Column #2
Column Information                           Agilent 19091P-MS4: 2
HP-PLOT Molesieve                            
Temperature Range                            -60 °C—300 °C (300 °C)
Dimensions                                   30 m x 320 μm x 12 μm
Column lock                                  Unlocked
In                                           Front SS Inlet N2
Out                                          Back Detector TCD
(Initial)                                    40 °C
Pressure                                     3.3447 psi
Flow                                         0.5 mL/min
Average Velocity                             11.38 cm/sec
Holdup Time                                  4.3936 min
Flow
Setpoint                                     On
(Initial)                                    0.5 mL/min
Post Run                                     1.7077 mL/min



Front Detector FID
                                             ***Excluded from Affecting GC's Readiness State***
Makeup                                       N2
Heater                                       On    250 °C
H2 Flow                                      Off
Air Flow                                     Off
Makeup Flow                                  On    5 mL/min
Carrier Gas Flow Correction                  Does not affect Makeup or Fuel Flow
Flame                                        Off

Back Detector TCD
                                             ***Excluded from Affecting GC's Readiness State***
Makeup                                       N2
Heater                                       On    200 °C
Reference Flow                               On    22 mL/min
Makeup Flow                                  On    8 mL/min
Filament                                     Off
Negative Polarity                            On

Signals
Signal #1: 
Description                                  None

Signal #2: 
Description                                  None

Signal #3: 
Description                                  None

Signal #4: 
Description                                  None




MS Information
-- -----------


General Information
------- -----------


Acquisition Mode         : Scan
Solvent Delay (minutes)  : 4.1
Tune file                : D:\MassHunter\GCMS\1\5977\etune.u
EM Setting mode Gain     : 1.000000

Normal or Fast Scanning  : Normal Scanning
Trace Ion Detection      : Off
Run Time (if MS only)    : 650 minutes 

[Scan Parameters]
Start Time               : 4.1
Low Mass                 : 30
High Mass                : 350
Threshold                : 50
A/D Samples:             : 8


[MSZones]

MS Source                   : 250 C   maximum 250 C
MS Quad                     : 150 C   maximum 200 C

Timed Events
----- ------
Number Events= 0



                     END OF MS ACQUISTION PARAMETERS


                        TUNE PARAMETERS for SN: US1837R013
                        ---------------------------------

 Trace Ion Detection is OFF.

    34.593   :   EMISSION     
    70.007   :   ENERGY       
     4.985   :   REPELLER     
    89.822   :   IONFOCUS     
    15.136   :   ENTRANCE_LENS
  1126.717   :   EMVOLTS      
                              1248.3  : Actual EMV 
                               1.00   : GAIN FACTOR 
  1387.000   :   AMUGAIN      
   136.500   :   AMUOFFSET    
     1.000   :   FILAMENT     
     0.000   :   DCPOLARITY   
    11.488   :   ENTLENSOFFSET
     8.002   :   Ion_Body     
    -0.098   :   EXTLENS      
  -545.000   :  MASSGAIN        
   -26.000   :  MASSOFFSET      

                        END OF TUNE PARAMETERS
                        ----------------------



                       END OF INSTRUMENT CONTROL PARAMETERS
                       ------------------------------------
