<?xml version="1.0" encoding="utf-8"?>
<srm_settings format_version="21.1" software_version="Skyline-daily (64-bit) 21.1.1.160 (55b1a57db)">
  <settings_summary name="Default">
    <peptide_settings>
      <enzyme name="Trypsin" cut="KR" no_cut="P" sense="C" />
      <digest_settings max_missed_cleavages="2" />
      <peptide_prediction use_measured_rts="true" measured_rt_window="2" />
      <peptide_filter start="25" min_length="7" max_length="50" auto_select="true">
        <peptide_exclusions />
      </peptide_filter>
      <peptide_libraries pick="library" />
      <peptide_modifications max_variable_mods="3" max_neutral_losses="1" internal_standard="none">
        <static_modifications>
          <static_modification name="Acetyl (N-term)" terminus="N" variable="true" formula="C2H2O" unimod_id="1" short_name="1Ac" />
          <static_modification name="Oxidation (M)" aminoacid="M" variable="true" formula="O" unimod_id="35" short_name="Oxi">
            <potential_loss formula="H4COS" massdiff_monoisotopic="63.998285" massdiff_average="64.10701" />
          </static_modification>
          <static_modification name="Carbamidomethyl (C)" aminoacid="C" formula="H3C2NO" unimod_id="4" short_name="CAM" />
          <static_modification name="Gln-&gt;pyro-Glu (N-term Q)" aminoacid="Q" terminus="N" variable="true" formula="-H3N" unimod_id="28" short_name="PGQ" />
          <static_modification name="Ammonia-loss (N-term C)" aminoacid="C" terminus="N" variable="true" formula="-H3N" unimod_id="385" short_name="dAm" />
          <static_modification name="Glu-&gt;pyro-Glu (N-term E)" aminoacid="E" terminus="N" variable="true" formula="-H2O" unimod_id="27" short_name="PGE" />
        </static_modifications>
        <heavy_modifications />
      </peptide_modifications>
    </peptide_settings>
    <transition_settings>
      <transition_prediction precursor_mass_type="Monoisotopic" fragment_mass_type="Monoisotopic" optimize_by="None" />
      <transition_filter precursor_charges="2,3,4,5,6" product_charges="1" precursor_adducts="[M+H]" product_adducts="[M+]" fragment_types="y,p" small_molecule_fragment_types="f,p" fragment_range_first="m/z &gt; precursor" fragment_range_last="3 ions" precursor_mz_window="0" auto_select="true">
        <measured_ion name="N-terminal to Proline" cut="P" sense="N" min_length="3" />
      </transition_filter>
      <transition_libraries ion_match_tolerance="0.5" min_ion_count="0" ion_count="3" pick_from="all" />
      <transition_integration integrate_all="true" />
      <transition_instrument min_mz="50" max_mz="3000" mz_match_tolerance="0.055" />
      <transition_full_scan precursor_isotopes="Count" precursor_isotope_filter="3" precursor_mass_analyzer="centroided" precursor_res="10" retention_time_filter_type="ms2_ids" retention_time_filter_length="5">
        <isotope_enrichments name="Default">
          <atom_percent_enrichment symbol="H'">0.98</atom_percent_enrichment>
          <atom_percent_enrichment symbol="C'">0.995</atom_percent_enrichment>
          <atom_percent_enrichment symbol="C&quot;">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="N'">0.995</atom_percent_enrichment>
          <atom_percent_enrichment symbol="O&quot;">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="O'">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="Cl'">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="Br'">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="P'">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="S&quot;">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="S'">0.99</atom_percent_enrichment>
          <atom_percent_enrichment symbol="H&quot;">0.99</atom_percent_enrichment>
        </isotope_enrichments>
      </transition_full_scan>
    </transition_settings>
    <data_settings document_guid="6dfa908d-2b83-478a-a77e-f335e23b7dea" audit_logging="true" />
  </settings_summary>
</srm_settings>