In our third webinar of 2025, over 153 participants joined Michael MacCoss (Principal Investigator) and Nick Shulman (Skyline Developer) on December 11 as they presented the newest tutorial in Skyline, Peak Boundary Imputation for DIA Data.

Mike presented a case for imputing boundaries for missing DIA data, and Nick provided the technique using Skyline to demonstrate the process.

The webinar concluded with a great Q&A session which generated a number of great questions and will lead to more discussion in a future webinar.

-- the Skyline Team

Michael MacCoss (Principal Investigator)

Presentation

Nick Shulman (Skyline Developer)

Tutorial

Data (?GB)

Dear Skyline Users,

Join us for an all-new Skyline Tutorial Webinar on Thursday, December 11, 2025, at 8:00 am (Pacific Time). This free webinar will explore new features in Skyline for imputing peak integration boundaries for missing peaks. This ensures all peak areas in your statistics are derived from the actual mass spec signal present in your data.

Webinar #27: Peak Boundary Imputation for DIA Data

[registration closed]

• Derive complete peak area matrices for statistical analysis from the signal in your DIA data
• Use retention time alignment to impute peak boundaries instead of statistical imputation
• Learn how to use and tune this new feature for your data and your needs

This webinar will include a brief introduction from Brendan MacLean, Skyline Principal Developer, a presentation from Michael MacCoss, Principal Investigator of the MacCoss lab, and a tutorial walk-through by Nick Shulman, Developer, Skyline team.

Join us, and learn how to improve your DIA data with Peak Boundary Imputation.

--Skyline Team

  Presenters

Michael MacCoss
(Principal Investigator)

Nick Shulman
(Developer)