This is the participant's page for the 3-day Targeted Proteomics Strategies Workshop which was held prior to the 2018 Lorne Proteomics Symposium ,  January 29 - 31, 2018 in at the University of Melbourne, Parkville, VIC, Australia. 

Schedule

Course Instructors

When:
Monday, Jan 29 from 9:30-5:30pm (50 min. for lunch at 12:40pm) 
Tuesday, Jan 30 from 8:30-6pm (45 min. for lunch at 1:50pm) 
Wednesday, Jan 31 from 9-5pm (1 hr. for lunch at 12:30)

Where:  
University of Melbourne
Arts West | North Wing Building (building 148) | Lectorial Room II (Room 556 - Level 5)
Royal Parade
Parkville, VIC 3052 (Transportion options and maps)

Additional information:
Available from the Pre-Lorne Workshop course page.

Course Content:

All Presentations in zip file

Pre-Lorne Flashdrive content (5.9GB)

SWATH Tutorial Data (7.8GB)

MSstats  Addendum - slides and data

The instructors have finalized their presentations and are posted below (Hands-on sessions had no slide presentations unless otherwise noted): 

Day 1

9:30 am Registration open and morning tea
10:00 am MacLean - Opening Remarks
10:40 am Spencer - Moving from Discovery to Targeted Proteomics
11:40 am MacLean - Lecture: Introduction to Skyline
12:40 pm - Lunch
1:30 pm MacLean - Hands-on: Targeted Method Editing
2:45 pm MacLean - Lecture: Targeted Method Refinement
4:00 pm - Break
4:30 pm Spencer - Lecture: Building Prior Knowledge for SRM Assays

Day 2
8:30 am MacLean - Hands-on: Data Analysis in Skyline
9:45 am Spencer - Lecture: Protein Quantification
10:45 am - Break
11:15 am MacLean - Hands-on: Group Comparison Statistics and Reports in Skyline
12:15 pm MacLean - Hands-on: Calibrated Quantification with Skyline
1:15 pm - Lunch
2:00 pm MacLean - Lecture: Overview and Keys to Success in Processing DDA data with Skyline
2:30 pm MacLean - Hands-on: Label-free Quantitative Analysis of DDA data with Skyline
4:00 pm - Break
4:30 pm Spencer - Hands-on: Parallel Reaction Monitoring (PRM) with Skyline

Day 3
9:00 am Searle - Lecture: Introduction to DIA
9:30 am Searle - Hands-on: Extracting Peptides from DIA in Skyline
10:30 am - Break
11:00 am Searle - Lecture: Details of DIA Acquisition
11:30 am Searle - Hands-on: Large-scale DIA with DDA Libraries in Skyline
12:30 pm - Lunch
1:30 pm Searle - Hands on: Analyzing DIA with Walnut/Skyline - Instructions
3:30 pm - Break
4:00 pm Aebersold - Design and Biological Relevance of Targeted Proteomic Experiments

-- Brendan MacLean
Course Organizer