Could skyline extract the LC area of isomers which separated by ion mobility sxu374  2022-09-12 12:58
 

Dear Skyline support,

I've used LC-IM-MS/MS to separate small molecule isomers. The isomers have the same m/z, retention time, and MS/MS spectra, they could be srperated by diffrernt drift time(CCS). I hope to calculate the ratio of these isomers according to their LC peak area, not the IMS spectra intensities or area. Could skyline extract the LC area of isomers which separated by ion mobility?

Looking forward to hearing from you!

Thanks,
Anne
 
 
Brian Pratt responded:  2022-09-12 14:27

Hi Anne,

Yes, certainly. You'll just need to give each isomer a distinct name and CCS value in your input transition list.

Thanks for using the Skyline support board!

Brian Pratt
 
sxu374 responded:  2022-09-12 14:36

Hi Dr. Pratt,

Thank you so much for the valuable response!

Best,
Anne