Number of MRM method channels for peptides containing two or more amino acids modified with MassLynx ryuta kobayashi  2022-07-04 21:28
 

I tried to export the method to MassLynx using Skyline, but the number of channels for peptides containing two or more modified amino acids was limited to five.
In such a case, is there a way to add 5 or more channels?

Kind regards,
Ryuta
 
 
Kaipo Tamura responded:  2022-07-07 12:20

Hi Ryuta,

Would you mind sharing your Skyline document and template method so we can try to reproduce the issue?
You can attach them to this support request or upload them here: https://skyline.ms/files.url

Thanks,
Kaipo
 
ryuta kobayashi responded:  2022-07-07 20:26

Hi Kaipo,

Thank you for your reply.
I send documents and files.
Please check.

Thanks,
Ryuta
 
Kaipo Tamura responded:  2022-07-07 22:51

Hi Ryuta,

The .skyl file actually only contains audit log information. You can share the Skyline document by going to File > Share... and sharing the resulting .zip file.

Thanks,
Kaipo
 
ryuta kobayashi responded:  2022-07-13 17:38

Hi Kaipo,

Sorry, I sent zip file.

Thanks,
Ryuta
 
Kaipo Tamura responded:  2022-07-14 10:39

Hi Ryuta,

Thanks for sharing those files. I will check with Waters about this issue and let you know when we have more information about it.

Thanks,
Kaipo
 
Kaipo Tamura responded:  2022-08-11 12:52

Hi Ryuta,

Waters has let us know that they have contacted you directly about this issue. We are also working with them to try to solve the issue for future versions.

Thanks,
Kaipo