Probably a newbie issue but I can't figure it out or find it in the support section. My spectra were working wonderfully - picking peaks, adjusting peak widths, giving dotp values and retention times (Great Program). I imported a raw data file and everything went to dotted lines and added precursor ions in my transition lists. I read that the dotted lines means they are now marked as non-quantitative but I checked and they are still marked quantitative (see attached). I started a new file, loaded a single peptide and the older libraries (without the raw file) and it is still dotted with no dotp or peak picking. I tried removing the precursor ions from the list and Refine/Reintegrate but no luck. What did I do wrong and how do I fix it?
Skyline 21.1.0.278