Build library from MSfragger output pepxml | af1234 | 2021-06-19 15:37 | |||||||||
I have a set of 16 synthetic peptides (heavy Cterm R/K) which I ran individually and as pool in DDA on a Qexactive and searched using MSfragger. I am now trying to import the results into Skyline to build targeted assays but it seems there are some issues. I tried the usual import DDA search to build a library but once I load the pepxml files there are not a correspondent MGF file and an error. I cannot generate a library within MSfragger because there are not enough peptides across all samples for RT alignment. Are there some preferred settings/tricks for import MSfragger output? Thanks! |
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