|Creation of spectral library using DIA without DDA||mjgou||2021-06-15|
Dear Skyline Team,
In our lab, we are currently using a 6560 Ion-Mobility qTOF mass spectrometer from Agilent for untargeted proteomics studies and Spectrum Mill is used to generate peptides/proteins identification lists. We are particularly interested in using Skyline to create and manage specific spectral libraries for our clinical applications. However, our workflow does not include the prior acquisitions in DDA to build the library due to the limited amount of samples. We would like to directly use the DIA acquisition files to create this library. Do you think this workflow could be compatible with Skyline?
Thanks in advance,