Thank you for uploading your raw file.
That file does not contain valid data. The file is 1.4GB in size, but all of the bytes in the file are "0".
Usually Skyline has no trouble reading Q-Exactive raw files, but this particular file does not contain the right data all. This corruption might have happened when the file was being copied from one computer to another. If you go back to the computer that is attached to your mass spectrometer you might find a copy of this file which has valid data in it.
With your help, I quickly found the reason for the above problems. As you said, it was my tool that copied the data that broke down and damaged the file. Sincerely thank you and your team for your timely reply, wish you well, thank you again!