" don't know how to read "when uploading dia data in raw format 1010419931  2021-02-24
 

Dear skyline development team, when using skyline's Data independent acquisition function, I encountered the " don't know how to read " prompt as shown in the figure when uploading dia data in raw format .I used Thermo Scientific's Q exictive HF to get the above data in raw format.I updated the skyline software, but it didn't solve the problem effectively.
What is the reason for this? How can I solve it?I hope I'm the first one to ask this question. I'm looking forward to your reply. Thank you.

 
 
Nick Shulman responded:  2021-02-24
Can you send us your raw file "dia1-60windows-02.20.raw"?

If that file is less than 50MB you can attach it to this support request.
Otherwise, you can upload it here:
https://skyline.ms/files.url

-- Nick
 
Nick Shulman responded:  2021-02-24
Thank you for uploading your raw file.

That file does not contain valid data. The file is 1.4GB in size, but all of the bytes in the file are "0".

Usually Skyline has no trouble reading Q-Exactive raw files, but this particular file does not contain the right data all. This corruption might have happened when the file was being copied from one computer to another. If you go back to the computer that is attached to your mass spectrometer you might find a copy of this file which has valid data in it.

-- Nick
 
1010419931 responded:  2021-02-24
Hi, Nick
With your help, I quickly found the reason for the above problems. As you said, it was my tool that copied the data that broke down and damaged the file. Sincerely thank you and your team for your timely reply, wish you well, thank you again!