How to use explicit retention time in proteomics interface? yoneyamat  2021-02-03

Dear skyline team,

I am trying to measure peptides in SRM mode, and would like to use an explicit retention time on the chromatogram view. The small molecule interface has a column to paste the explicit retention time. Edit>Inset>Transition List. However, there is no column in proteomics interface to paste the explicit retention time for each peptides.
I would like to know how to use explicit retention time in proteomics interface.

Thank you in advance for your help.

Brian Pratt responded:  2021-02-03

You can add those in the Document Grid by adding the appropriate column in a custom report, then pasting them in.

Thanks for using the Skyline support board,

Brian Pratt

Brendan MacLean responded:  2021-02-03

If you are not familiar with custom reports, you can learn more from this tutorial:

yoneyamat responded:  2021-02-04

Hello Brendan and Brian,

Thank you for your quick and specific advice!
I finally succeeded in displaying the explicit retention time in the proteomics interface based on your advice!

Best regards.