How to use explicit retention time in proteomics interface?

yoneyamat

2021-02-03 00:53

Dear skyline team,

I am trying to measure peptides in SRM mode, and would like to use an explicit retention time on the chromatogram view. The small molecule interface has a column to paste the explicit retention time. Edit>Inset>Transition List. However, there is no column in proteomics interface to paste the explicit retention time for each peptides.
I would like to know how to use explicit retention time in proteomics interface.

Thank you in advance for your help.

Brian Pratt responded:	2021-02-03 15:32
You can add those in the Document Grid by adding the appropriate column in a custom report, then pasting them in. Thanks for using the Skyline support board, Brian Pratt

Brendan MacLean responded:	2021-02-03 15:42
If you are not familiar with custom reports, you can learn more from this tutorial: https://skyline.ms/tutorial_custom_reports.url

yoneyamat responded:	2021-02-04 23:49
Hello Brendan and Brian, Thank you for your quick and specific advice! I finally succeeded in displaying the explicit retention time in the proteomics interface based on your advice! Best regards.