"Failed importing results...The calculator ... requires all of its standared peptides in order to determine a regression | Mark Athanason | 2020-10-05 08:35 | |||||||||||||||
Hello, I'm getting the following error, seen below. I'm simply trying to build a spectral library to perform DIA quant with 2 DDA runs. I've never seen this error before. I'm using the most current version of skyline daily. For RT standards, I selected automatic because when I select biognosys 10 or 11 it doesn't find any standards, for some reason. When selecting automatic, it finds 10 standards with a r^2 = 1. Going down the wizard is fine, then when it's importing the DDA data it cancels and throws this error: At 11:25 AM: The search was performed with comet with .RAW files converted to .mzXML with default settings. The output file is a pep.xml. I think the error is occurring when it's trying to automatically train the mProphet model. In the search settings I performed a concatenated decoy search, with skyline being told to make 1 reverse decoy to train mProphet. |
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