Targeted Calibration Curve Value Import gsbyram  2020-01-06
 

Hey Everyone,

I have a targeted steroid assay which quantifies roughly 35 compounds. The compounds contained in the calibration curve have different concentrations based around their reference range. Is there a way to individually assign concentration values for each compound in a calibration standard instead of a blanket value for all compounds?

Best,
Gregory
West Coast Metabolomics Center
UC Davis
Oliver Fiehn Laboratory

 
 
Nick Shulman responded:  2020-01-06
Currently there is no way to do this in Skyline.

If your different compounds vary in a simple way between each other, then you can make use of the "Concentration Multiplier" on Molecules/Peptides.

But if the spiked in concentrations are varying in a complex way across molecules and replicates, such that you need to specify the "full matrix" of concentrations, there is currently no way to do this in Skyline.

This question gets asked every few months on the support board. You might find some more information here:
https://skyline.ms/announcements/home/support/thread.view?rowId=43036

-- Nick