Hi Christina,
I was able to construct a transition list from your excel sheet without too much difficulty. For example:
"Precursor Name" "Precursor Adduct" "Explicit Retention Time" "Precursor Charge" "Explicit Retention Time Window" "Precursor m/z"
C10:0 [M+] 4.683 1 0.1 186.2
C10:0 [M(-112.1)+] 4.683 1 0.1 74.1
C10:0 [M(-99.1)+] 4.683 1 0.1 87.1
C10:0 [M(-43.1)+] 4.683 1 0.1 143.1
C10:0 [M(-131.1)+] 4.683 1 0.1 55.1
C10:0 [M(-31.1)+] 4.683 1 0.1 155.1
It was necessary to add that first line "C10:0 [M+] 4.683 1 0.1 186.2" to establish the mass of the no-losses precursor.
I constructed the adducts for the other lines using the formula =CONCATENATE("[M(",F3-I3,")+]") where column F is your "Product Ion" and column I is your "Molecular Ion".
Let me know how that works out for you!
Thanks for using the Skyline support board,
Brian Pratt