Problems with method export hober  2019-12-09
 

Hi,
I'm using Skyline for screening of recombinant proteins for method development on our SRM instrument (TSQ Altis).
In this workflow, the method export has worked really well most of the time. I have a Skyline document with all my targets in the target list and then export my methods using One method per protein, since my proteins are in independent samples. Occasionally, when I have longer peptides, the collision energy which Skyline tries to write to the method files exceeds the collision energy possible on the instrument (<65). This is especially a problem when doing the CE optimization. This causes the entire methods export to abort and I cannot even retrieve files for the proteins which don't cause the error.

Would it be possible to introduce an upper limit in regards to collision energy?

Best,
/Andreas