Export protein abundance from MRM data benoit fatou  2019-12-03
 

Dear Skyline Team,

I was wondering if there is a way to export the protein abundance in Skyline based on the average of the peptide areas.
Thanks,
Benoit

 
 
Nick Shulman responded:  2019-12-03
Skyline has a feature in the Document Grid called the "Pivot Editor" which lets you group rows together based on the values in some columns and summarize the rest of the columns using operations such as Mean or Sum.

Here is the page with documentation about the Pivot Editor
https://skyline.ms/wiki/home/software/Skyline/page.view?name=PivotEditor

There is a document on there which describes how to calculate the CV of peak areas. I hope there is enough information in there so that it will be easy to see how to go about calculating the average peptide area across proteins and replicates.
If anything is not clear let me know and I will try to create a document that is more specific for your scenario.
-- Nick