Transiton Results Report Khizra  2019-09-13
 

Hello Skyline Team,

I would greatly appreciate if you could help provide some explanation to aid my understanding of the Transition Results Report. It includes multiple rows for the each fragment ion of each protein and each row has a different retention time. It is also omitting some fragment ions from the table. Can you please provide some information on firstly why it is omitting some ions from the report, and secondly why there are multiple rows each with a different RT for each ion displayed. Please review the attached file which shows as example.

Thank you,
Khizra.

 
 
Nick Shulman responded:  2019-09-13
Khizra,

The Transitions Results report has a separate row for each transition and each replicate in your Skyline document. You have three replicates in your Skyline document. For this reason, each transition is going to appear three times in the Transition Results report, and the Retention Time, Area etc columns will be different across the replicates.

If you would like to learn more about the Document Grid you should take a look at the Custom Reports tutorial:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_custom_reports

I am not sure why your y9 ion is not showing up in the report. If you send us your Skyline document I can take a look.

In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms.

If that .zip file is less than 50MB you can attach it to this support request.
Otherwise, you can upload it here:
https://skyline.ms/files.url

-- Nick