Not able to see libray ion in the result file and how to do the quantitation of the peaks drrenugoel  2019-08-23 07:21
 

Kindly see the attached file. I have built the library in the peptide setting tab. However, I am not able to see the library ions in the attached file (skyline_doubt). some setting has been changed as I used to get it. Kindly see the attached (skyline2).

I have run these samples on 5600 sciex in MRM-HR mode and now want to do the quantitation. Could you please let me know how to take it out peak area for the same.
 
 
Brendan MacLean responded:  2019-08-23 07:51

You should be able to switch back with View (or right-click) > Transitions > All. It appears you are currently in View > Transitions > Total mode.

Thanks for posting to the support board and supplying the screenshots.

--Brendan
 
drrenugoel responded:  2019-08-23 08:06

Thank you so much. fantastic. I had run 5 biological replicates from three conditions. I would like to do the quantitation. Could you please help?
 
Brendan MacLean responded:  2019-08-24 21:28

This is probably a good Webinar for you:

https://skyline.ms/webinar08.url

And this tutorial:

https://skyline.ms/tutorial_grouped.url