Variable modification dawn dufield  2019-08-05

How do you add a modification that you do not know which AA it goes on. For example a ribosylation that I know adds a certain MW, but fall off in the MS so doesn't change the fragments? or how do you look for a modification that can be on any AA's without creating it on all possible combinations.. Can you generate a list of the possible MRM's?


Brendan MacLean responded:  2019-08-05

All possible through Edit > Modify Peptide and neutral losses.

There is a webinar on peptide modifications that may help.