|Global setting / adjustment of peak integration boundaries?||Matthias||2019-07-08|
I was trying to get an idea of how reliable the quantification of peptides/proteins using different DIA Methods would be.
Using the Pivot Editor I focused on proteins with a max Detected Q value below 0.01 (see attached pdf showing screenshots).
Although the values show the expected trend (higher values with shorter cycle time due lower max IT). The average values between 14 -16 seem to be a little high.
When going through the data I noticed that the integration boundaries for some peptides are nicely selected (e.g. AIGPHDVLATLLNNLK) but on the other hand for some peptides the integration boundaries seem to be too broad/ unspecific selected (e.g. LQTSSVLVSGLR).
Is there a way to specify global settings for peak integrations boundaries, e.g. more conservative or less conservative / more sensitive? Or does one have to go through all peptides manually and adjust the boundaries?