Protein relative quantification | tiziano brogna | 2019-07-03 04:01 | |||||||||
Hi, I have 2 biological conditions for which we monitore multiple proteins to check if they are up or down-regulated. I would like to know how Skyline consider the variability for the different peptides used for protein relative quantification (at the end we obtain fold change ratio with error bar that are linked with the analytical replicates if I understood well)? I based the method on this tutorial: In other word, I have the variability for the analytical replicates and now I would like to know how Skyline use the peptides signals to give the fold change information for the 2 conditions tested. Thank you, Best regards, Tiziano |
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