|Retention Time Calibration for DIA-data||yb77618||2019-05-17|
Hi Skyline Team:
I have DIA-MS data and pre-built assay library, so I want to use Skyline-Daily for data analysis.
The assay library was loaded successfully, 11 iRT-peptides were chosen as standard for retention time calibration.
However, I found out the criteria for DIA-MS data loading was so stringent, 80% peptides were needed with a correlation of 0.99 in regression.
Is that possible for us to change the requirements for calibration? Like to calibrate by hands or lower the R-square value?
(I want to remove some iRT-peptides for better correlation in the regression, an alert popped up, see attached image)
Thank you in advance for answering my questions.