integration Frederique van Holthoon  2019-02-06 02:04
 

I'm just starting to do a little bit more with skyline. I succeeded in importing raw data files. Everything looks pretty good, however some integrations/IDs look strange (see attached file). What is going wrong, how do I fix it?
With kind regards
Frederique

 
 
Nick Shulman responded:  2019-02-06 02:50
The vertical lines with the letters "ID" above them indicate times from your spectral library where that peptide was identified in that raw file.
If you want those lines to disappear you can right-click on the chromatogram and choose "Peptide ID Times > None".

Is that what you are asking about? There are a lot of those vertical lines, which probably does mean that there were many MS2 scans in your raw file where that particular peptide was identified.
-- Nick