"Peak Areas" graph

"Peak Areas" graph k valgepea  2018-05-23

Dear Skyline team,

I hope you can help me with two short questions:

  1. I am double-checking if it is somehow possible to plot the "rdotp" value for a precursor above the bar showing its peak area in the "Peak Areas - Replicate comparison" graph? I can plot "dotp" in my Skyline version (, but not "rdotp".

  2. Could I simultaneously have two "Peak Areas - Replicate comparison" graphs opened within one Skyline document? For example, one grouped by "Replicate" and showing non-normalised peak areas while the other grouped by "Condition" and showing CVs of pear areas normalised to heavy?

Thanks in advance!

Brendan MacLean responded:  2018-06-22

Nice ideas. Thanks for contributing them, but neither is yet possible. Sorry.

roman sakson responded:  2021-08-07

Hi Brendan and the team,

I was just browsing the Support board, looking for something else and came across this older thread. I assume it is still not possible to display the rdotp in the Peak Areas window? I agree with the person who opened the thread that it would be a very nice feature to have. At the moment my workaround is to put the results grid containing rdotp values below the Peak Areas graph, when I am reviewing my peptide data.

Thank you for considering it,

Brendan MacLean responded:  2021-08-11

Okay. I have elevated this to a TODO issue on the issues board and given it to Rita to hopefully implement this cycle.


Thanks for the feedback, Roman and Kaspar.


roman sakson responded:  2021-08-11

So cool, thank you! Looking forward to it.

I have another question, also related to the Peak Areas graph so maybe I can quickly post it here as well: when I normalize to total, I am getting the peak area percentage on the y axis. Is there a way in Skyline to display the percentage values in the document grid? I did not manage to get it via any normalized area parameter, at the moment the only way for me to access the values directly seems to copy data from the graph via mouse right click and to paste it somewhere else...

Thanks for your support,