Hi Kathrin,
That is not a very helpful error code, sorry. I think the issue is that Skyline wants to create a temporary file in the same directory as the .raw, but the .raw isn't actually in a directory at all.
Try copying the .raw to a local drive and I think it will be fine.
Meanwhile we'll try to make that error message more helpful.
Thanks for using the Skyline support board!
Brian Pratt |
Hi, Kathrin,
Can you press the "more info" button and tell us what is says (when you get an error during chromatogram extraction, the "more info" button is a checkbox at the bottom of the "Importing Results" status window). That will tell us exactly where in the code this error is being encountered.
You would definitely get an error if your .sky file was saved in directory that you did not have write permission on, but my guess is that that is not what is going on here. |
Hi,
the whole error message is:
At 08:37:
Failed importing results file '\\eu.boehringer.com\users\bib\users1\wengerk\Desktop\20180411_tMSMS_Z2u3_KW_undepl_101.raw'.
Win32 Error: 3
Inner exceptions:
Exception type: System.IO.IOException
Error message: Win32 Error: 3
Win32 Error: 3
at pwiz.Skyline.Util.FileStreamManager.GetTempFileName(String basePath, String prefix, UInt32 unique) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Util\UtilIO.cs:line 776
at pwiz.Skyline.Model.Results.ChromGroups.SpillFile.<>c__DisplayClass1.<CreateFileStream>b__0() in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Model\Results\ChromCollector.cs:line 659
at pwiz.Skyline.Util.Helpers.Try[TEx](Action action, Int32 loopCount, Int32 milliseconds) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Util\Util.cs:line 1821
at pwiz.Skyline.Model.Results.ChromGroups.SpillFile.CreateFileStream(String cachePath) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Model\Results\ChromCollector.cs:line 657
at pwiz.Skyline.Model.Results.BlockedList`1.Add(Int32 chromatogramIndex, TData data, BlockWriter writer) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Model\Results\ChromCollector.cs:line 202
at pwiz.Skyline.Model.Results.ChromDataCollectorSet.ProcessExtractedSpectrum(Single time, Collectors chromatograms, Int32 scanId, ExtractedSpectrum spectrum, Action`2 addCollector) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 1376
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.ProcessSpectrumList(MsDataSpectrum[] spectra, ChromDataCollectorSet chromMap, Single rt, SpectrumFilter filter, String scanId) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 473
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.ExtractChromatogramsLocked() in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 313
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.ExtractChromatograms() in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 215
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.SetRequestOrder(IList`1 chromatogramRequestOrder) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 551
at pwiz.Skyline.Model.Results.ChromCacheBuilder.Read(ChromDataProvider provider) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 409
at pwiz.Skyline.Model.Results.ChromCacheBuilder.BuildCache() in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 270
The files are saved on my desktop. So the computer should have writing permision.
Thanks for the quick help :) |