Hi,

I encounter this problem in both PRM analysis and MRM method setup when i was trying to look for a product ion which can have more than one neutral loss (for example, a y7 ion with two phosphorylation sites). The peptide and transition settings can predict the product ion with either one of the neutral loss (y7-98), but not both (y7-196). I was just wondering is there a way that we can have the skyline do that, or we can manually put in a product ion with both neutral loss (-196) in the list (especially for PRM analysis).

Thanks very much in advance!

Lorrain