Import Peptide Error | rbao1357 | 2018-02-06 16:23 | |||||||||||||||||||||||||||||||||
Hi guys, I am a new user of Skyline (and to the MS field). I have been experiencing an error over the last 2 weeks when attempting to import peptides in the initial steps. I have previously created technical replicate files under names (RB1, RB1b, RB1c etc), under the (.group), (wiff), (.wiff.scan) formats. These files are all stored in a single folder. When attempting to extract chromatograms, the peptide search is duplicating the first (RB1.wiff) file, rather than finding (RB1b etc) files. I have tested this with a number of different files, and the software is not locating the other files under different file names. I have also tested against (RB1) and (RB2) only, and it successfully builds the peptide spectrum. This generates the below error. An item with the same key has already been added. Skyline version: 4.1.0.11714 (64-bit) Installation ID: cf11b43b-5d9e-48e1-b3b6-ba6bcb35d288 Exception type: ArgumentException Error message: An item with the same key has already been added. -------------------- Stack trace: at System.ThrowHelper.ThrowArgumentException(ExceptionResource resource) at System.Collections.Generic.Dictionary`2.Insert(TKey key, TValue value, Boolean add) at pwiz.Skyline.Model.Results.MeasuredResults.set_Chromatograms(IList`1 value) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Model\Results\MeasuredResults.cs:line 80 at pwiz.Skyline.Model.Results.MeasuredResults..ctor(IList`1 chromatograms, Boolean disableJoining) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Model\Results\MeasuredResults.cs:line 57 at pwiz.Skyline.SkylineWindow.ImportResults(SrmDocument doc, List`1 namedResults, String optimize) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\SkylineFiles.cs:line 2601 at pwiz.Skyline.SkylineWindow.ModifyDocumentInner(Func`2 act, Action onModifying, Action onModified) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Skyline.cs:line 671 at pwiz.Skyline.SkylineWindow.ModifyDocument(String description, IUndoState undoState, Func`2 act, Action onModifying, Action onModified) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Skyline.cs:line 635 at pwiz.Skyline.SkylineWindow.ModifyDocument(String description, Func`2 act) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Skyline.cs:line 629 at pwiz.Skyline.FileUI.PeptideSearch.ImportPeptideSearchDlg.WizardFinish() in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\FileUI\PeptideSearch\ImportPeptideSearchDlg.cs:line 606 at pwiz.Skyline.FileUI.PeptideSearch.ImportPeptideSearchDlg.NextPage() in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\FileUI\PeptideSearch\ImportPeptideSearchDlg.cs:line 358 at System.Windows.Forms.Control.OnClick(EventArgs e) at System.Windows.Forms.Button.OnClick(EventArgs e) at System.Windows.Forms.Button.OnMouseUp(MouseEventArgs mevent) at System.Windows.Forms.Control.WmMouseUp(Message& m, MouseButtons button, Int32 clicks) at System.Windows.Forms.Control.WndProc(Message& m) at System.Windows.Forms.ButtonBase.WndProc(Message& m) at System.Windows.Forms.Button.WndProc(Message& m) at System.Windows.Forms.NativeWindow.Callback(IntPtr hWnd, Int32 msg, IntPtr wparam, IntPtr lparam) Exception caught at: at System.Windows.Forms.Application.ThreadContext.OnThreadException(Exception t) at System.Windows.Forms.Control.WndProcException(Exception e) at System.Windows.Forms.NativeWindow.Callback(IntPtr hWnd, Int32 msg, IntPtr wparam, IntPtr lparam) at System.Windows.Forms.UnsafeNativeMethods.DispatchMessageW(MSG& msg) at System.Windows.Forms.UnsafeNativeMethods.DispatchMessageW(MSG& msg) at System.Windows.Forms.Application.ComponentManager.System.Windows.Forms.UnsafeNativeMethods.IMsoComponentManager.FPushMessageLoop(IntPtr dwComponentID, Int32 reason, Int32 pvLoopData) at System.Windows.Forms.Application.ThreadContext.RunMessageLoopInner(Int32 reason, ApplicationContext context) at System.Windows.Forms.Application.ThreadContext.RunMessageLoop(Int32 reason, ApplicationContext context) at System.Windows.Forms.Form.ShowDialog(IWin32Window owner) at pwiz.Skyline.SkylineWindow.ShowImportPeptideSearchDlg(Nullable`1 workflowType) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\SkylineFiles.cs:line 2866 at pwiz.Skyline.SkylineWindow.importPeptideSearchMenuItem_Click(Object sender, EventArgs e) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\SkylineFiles.cs:line 2853 at System.Windows.Forms.ToolStripItem.RaiseEvent(Object key, EventArgs e) at System.Windows.Forms.ToolStripMenuItem.OnClick(EventArgs e) at System.Windows.Forms.ToolStripItem.HandleClick(EventArgs e) at System.Windows.Forms.ToolStripItem.HandleMouseUp(MouseEventArgs e) at System.Windows.Forms.ToolStrip.OnMouseUp(MouseEventArgs mea) at System.Windows.Forms.ToolStripDropDown.OnMouseUp(MouseEventArgs mea) at System.Windows.Forms.Control.WmMouseUp(Message& m, MouseButtons button, Int32 clicks) at System.Windows.Forms.Control.WndProc(Message& m) at System.Windows.Forms.ToolStrip.WndProc(Message& m) at System.Windows.Forms.ToolStripDropDown.WndProc(Message& m) at System.Windows.Forms.NativeWindow.Callback(IntPtr hWnd, Int32 msg, IntPtr wparam, IntPtr lparam) at System.Windows.Forms.UnsafeNativeMethods.DispatchMessageW(MSG& msg) at System.Windows.Forms.UnsafeNativeMethods.DispatchMessageW(MSG& msg) at System.Windows.Forms.Application.ComponentManager.System.Windows.Forms.UnsafeNativeMethods.IMsoComponentManager.FPushMessageLoop(IntPtr dwComponentID, Int32 reason, Int32 pvLoopData) at System.Windows.Forms.Application.ThreadContext.RunMessageLoopInner(Int32 reason, ApplicationContext context) at System.Windows.Forms.Application.ThreadContext.RunMessageLoop(Int32 reason, ApplicationContext context) at pwiz.Skyline.Program.Main(String[] args) in c:\proj\skyline_4_1_x64\pwiz_tools\Skyline\Program.cs:line 311 I have attached screenshots of the way the Skyline is behaving in a pptx. Are you able to provide any insight? Thanks, Rebecca |
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