Combined analysis

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Combined analysis AnnaKwasnik  2017-10-19 02:53
 
Hi, can anyone advice? I am combining different methods into one method in Skyline and the problem that I am facing is match of the data to the spectra libraries. Each method was developed using different spectra library and while the methods work well for the specific library they do not when I combine them into one Skyline file, the dotp for some of the peptides is lower in combined file. It seems like top library have the spectra for that peptide but its transitions were optimised on different library. Does anyone know if I could overcome this problem and selectively select the libraries for this combine Skyline file or do I have to keep and analyse methods separately?

Thank you for any help,

Anna
 
 
Brendan MacLean responded:  2017-10-19 16:44
Hi Anna,
You could use File > Share - Minimal to save your documents in a way that their libraries only contain spectra for the peptides they target. Then when you combine the documents, presumably, you would not see any cases where stray peptide spectrum matches from one library match to peptides in the wrong document. That is if you have documents.

A. with target peptide: APEPTIDER and library A containing spectra matching APEPTIDER AND CPEPTIDER
B. with target peptide: CPEPTIDER and library B containing spectra matching APEPTIDER AND CPEPTIDER

Then simply combining them will have the problem you highlight, where the combined document will use spectra for both target peptides from library A. However, if you first File > Share - Minimized, then you will end up with:

A. with target peptide: APEPTIDER and library A containing spectra matching APEPTIDER
B. with target peptide: CPEPTIDER and library B containing spectra matching CPEPTIDER

And combining them will no longer have this issue.

Would that work for you? If not, please explain. Thanks for posting your issue to the support board.

--Brendan
 
AnnaKwasnik responded:  2017-12-17 12:15
Thanks a lot Brendan for this clear explanation. Indeed, doing this way worked perfectly!