|HCD nCE used||fredrik edfors||2015-09-08|
Dear Skyline Team,
I'm trying to optimize the collision energy for a larger set of peptides that we want to analyze using a PRM-method on a QEHF. It would be really nice if you could find the HCD energy used for each spectra in the "Full scan"-window when using Skyline. I'm currently working with both Skyline and Xcalibur in parallel, trying to figure out what spectra (from the raw file where I can find the nCE used for that specific spectra) that is corresponding to the extracted chromatogram visualized in Skyline.
It would also help a lot if you would be able to visualize spectra in the "Full-scan"-window with the "View>Transitions>Total" option.
Thanks a lot,