Brian Pratt responded: |
2016-06-13 10:23 |
Hi Anna,
I'd like to take a look at that data.
Can you please send me the raw file, and the Skyline document that shows it properly loaded? Use File|Share|Complete to create a zip file which contains the Skyline document and all supporting documents.
I'll contact you privately about arranging the transfer.
Thanks
Brian Pratt |
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perzanowska an responded: |
2016-07-27 07:49 |
Hi Brian,
Did you get my email with requested files?
Thank you,
Anna |
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Brendan MacLean responded: |
2016-07-27 09:09 |
Hi Anna,
Brian is currently away on vacation. He will be back in the lab next week. Sorry to leave you hanging.
--Brendan |
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Brian Pratt responded: |
2016-08-02 12:58 |
Hi Anna,
Apologies for dropping the ball on this. I'm not sure what happened there.
Looking at the data, it seems that there just isn't much intensity for that ion in this replicate (I used the SeeMS program from ProteoWizard to inspect the data outside of Skyline.)
I'm not sure there are actually peaks to be found there?
Thanks
Brian |
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perzanowska an responded: |
2016-08-10 08:49 |
Hi Brian,
Recently, I’ve sent you two Skyline files that indicate that when I open the old Skyline files (saved before ver. 3.5.0.9319) the chromatograms for the peptide appear correctly however after re-import of the data the chromatograms disappear – I hope you have received this answer?
Now, as one of our computers has still the old version of Skyline (2.6.07176) I imported the data that in Skyline version 3.5.0.9319 caused the described issues and I found out that the data was imported correctly and chromatograms for that peptide appeared normally.
Thank you for your help.
Anna |
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Brendan MacLean responded: |
2016-08-10 22:20 |
Hi Anna,
Brian is out this week, but I am sure he will be back in touch early next week. Sorry for the delay.
--Brendan |
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Brian Pratt responded: |
2016-09-02 17:30 |
Just leaving a note to say that we worked this out off-list, and the issue should be resolved in the next Skyline-Daily. |
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