Skyline version: 21.0.9.118-d51872363 (64-bit) Installation ID: 96b4ab8f-321a-4d03-9f50-8c487ce27ffc Exception type: InvalidDataException Error message: The mass 0,329851410172327 of the custom molecule is less than the minimum of 5. -------------------- System.IO.InvalidDataException: The mass 0,329851410172327 of the custom molecule is less than the minimum of 5. ---> System.IO.InvalidDataException: The mass 0,329851410172327 of the custom molecule is less than the minimum of 5. at pwiz.Skyline.Model.CustomMolecule.Validate() in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\CustomMolecule.cs:line 529 at pwiz.Skyline.Model.AbstractModificationMatcher.TransitionFromPeakAndAnnotations(LibKey key, TransitionGroupDocNode nodeGroup, Adduct fragmentCharge, MI peak, Nullable`1 rank) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\AbstractModificationMatcher.cs:line 539 at pwiz.Skyline.Model.AbstractModificationMatcher.GetSmallMoleculeFragments(LibKey key, TransitionGroupDocNode nodeGroupMatched, SpectrumPeaksInfo spectrum, IList`1 transitionDocNodes) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\AbstractModificationMatcher.cs:line 502 at pwiz.Skyline.Model.AbstractModificationMatcher.CreateDocNodeFromSettings(LibKey key, Peptide peptide, SrmSettingsDiff diff, TransitionGroupDocNode& nodeGroupMatched) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\AbstractModificationMatcher.cs:line 481 at pwiz.Skyline.Model.LibKeyModificationMatcher.GetModifiedNode(LibKey key, SrmSettings settings, SrmSettingsDiff diff) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\LibKeyModificationMatcher.cs:line 335 at pwiz.Skyline.SettingsUI.ViewLibraryPepMatching.AssociateMatchingPeptide(ViewLibraryPepInfo pepInfo, Adduct charge, SrmSettingsDiff settingsDiff) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\SettingsUI\ViewLibraryPepMatching.cs:line 350 at pwiz.Skyline.SettingsUI.ViewLibraryPepMatching.MatchAllPeptides(ILongWaitBroker broker) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\SettingsUI\ViewLibraryPepMatching.cs:line 164 at pwiz.Skyline.SettingsUI.ViewLibraryPepMatching.AddAllPeptidesToDocument(ILongWaitBroker broker, AuditLogEntryCreatorList entryCreators) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\SettingsUI\ViewLibraryPepMatching.cs:line 92 at pwiz.Skyline.Controls.LongWaitDlg.RunWork(Action`1 performWork) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 254 --- End of inner exception stack trace --- at pwiz.Skyline.Util.Helpers.WrapAndThrowException(Exception x) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Util\Util.cs:line 1936 at pwiz.Skyline.Controls.LongWaitDlg.PerformWork(Control parent, Int32 delayMillis, Action`1 performWork) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 202 at pwiz.Skyline.SettingsUI.ViewLibraryDlg.AddAllPeptides(Boolean addLibraryToDocSeparately) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\SettingsUI\ViewLibraryDlg.cs:line 1616 at pwiz.Skyline.SettingsUI.ViewLibraryDlg.btnAddAll_Click(Object sender, EventArgs e) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\SettingsUI\ViewLibraryDlg.cs:line 1574 at System.Windows.Forms.Control.OnClick(EventArgs e) at System.Windows.Forms.Button.OnClick(EventArgs e) at System.Windows.Forms.Button.OnMouseUp(MouseEventArgs mevent) at System.Windows.Forms.Control.WmMouseUp(Message& m, MouseButtons button, Int32 clicks) at System.Windows.Forms.Control.WndProc(Message& m) at System.Windows.Forms.ButtonBase.WndProc(Message& m) at System.Windows.Forms.Button.WndProc(Message& m) at System.Windows.Forms.NativeWindow.Callback(IntPtr hWnd, Int32 msg, IntPtr wparam, IntPtr lparam) Exception caught at: at System.Windows.Forms.Application.ThreadContext.OnThreadException(Exception t) at System.Windows.Forms.Control.WndProcException(Exception e) at System.Windows.Forms.NativeWindow.Callback(IntPtr hWnd, Int32 msg, IntPtr wparam, IntPtr lparam) at System.Windows.Forms.UnsafeNativeMethods.DispatchMessageW(MSG& msg) at System.Windows.Forms.UnsafeNativeMethods.DispatchMessageW(MSG& msg) at System.Windows.Forms.Application.ComponentManager.System.Windows.Forms.UnsafeNativeMethods.IMsoComponentManager.FPushMessageLoop(IntPtr dwComponentID, Int32 reason, Int32 pvLoopData) at System.Windows.Forms.Application.ThreadContext.RunMessageLoopInner(Int32 reason, ApplicationContext context) at System.Windows.Forms.Application.ThreadContext.RunMessageLoop(Int32 reason, ApplicationContext context) at pwiz.Skyline.Program.Main(String[] args) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Program.cs:line 307