Parameters for C:\MassLynx\32-REC-21-CCS.PRO\ACQUDB\HDMSPFAS_CCS.EXP Created by 4.1 SCN957 Lock Spray Configuration: Reference Scan Frequency(sec) 30.000 Reference Cone Voltage(V) 40.000 Reference Trap Collision Energy 4.000 Reference DRE Setting 99.900 Temperature Correction: Temperature Correction Disabled Instrument Configuration: Lteff 3600.0 Veff 7224.29 Resolution 30000 Min Points in Peak 2 Acquisition Device WatersADC Acquisition Algorithm ADC Mode ADC Trigger Threshold (V) -1.00 ADC Input Offset (V) -1.56 Average Single Ion Intensity 17 ADC Amplitude Threshold 3 ADC Centroid Threshold -1 ADC Ion Area Threshold 0 ADC Ion Area Offset 10 ADC Pushes Per IMS Increment 1 EDC Delay Coefficient 1.4100 EDC Delay Offset 0.4000 Experimental Instrument Parameters Instrument Parameter Filename C:\MassLynx\32-REC-21-CCS.PRO\ACQUDB\FullscanHDMSPFAS_9.ipr (MODIFIED) Polarity ES- Capillary (kV) 2.5000 Source Temperature (°C) 150 Sampling Cone 28.0000 Source Offset 40.0000 Source Gas Flow (mL/min) 0.00 Desolvation Temperature (°C) 450 Cone Gas Flow (L/Hr) 50.0 Desolvation Gas Flow (L/Hr) 350.0 Nebuliser Gas Flow (Bar) 6.0 LM Resolution 5.0 HM Resolution 15.0 Aperture 1 0.0 Pre-filter 2.0 Ion Energy 0.8 Manual Trap Collision Energy FALSE Trap Collision Energy 4.0 Manual Transfer Collision Energy FALSE Transfer Collision Energy 2.0 Manual Gas Control TRUE Trap Gas Flow (mL/min) 0.00 HeliumCellGasFlow 180.00 IMS Gas Flow (mL/min) 100.00 Detector 2866 DetectorCache 2200 Sample Infusion Flow Rate (µL/min) 5 Sample Flow State LC Sample Fill Volume (µL) 250 Sample Reservoir C LockSpray Infusion Flow Rate (µL/min) 50 LockSpray Flow State Infusion LockSpray Reservoir B LockSpray Capillary (kV) 2.5 Use Manual LockSpray Collision Energy FALSE Collision Energy 4.0 Acceleration1 20.0 Acceleration2 0.0 Aperture2 5.0 Transport1 40.0 Transport2 20.0 Steering 0.00 Tube Lens 30 Pusher 1900.0 Pusher Offset 0.00 Puller 1370.0 Pusher Cycle Time (µs) Automatic Pusher Width (µs) Automatic Collector 60 Collector Pulse 10.0 Stopper 10 Stopper Pulse 20.0 Entrance 17 Static Offset 180 Puller Offset 0.00 Reflectron Grid (kV) 1.349 Flight Tube (kV) 10.00 Reflectron (kV) 4.140 Use Manual Trap DC TRUE Trap DC Entrance 3.0 Trap DC Bias 40.0 Trap DC 0.0 Trap DC Exit 0.0 Use Manual IMS DC TRUE IMS DC Entrance 20.0 Helium Cell DC 50.0 Helium Exit -20.0 IMSBias 2.0 IMS DC Exit 0.0 USe Manual Transfer DC TRUE Transfer DC Entrance 5.0 Transfer DC Exit 3.0 Trap Manual Control ON Trap Wave Velocity (m/s) 311 Trap Wave Height (V) 4.0 IMS Manual Control ON IMS Wave Velocity (m/s) 1000 IMS Wave Height (V) 40.0 Transfer Manual Control ON Transfer Wave Velocity (m/s) 219 Transfer Wave Height (V) 4.0 Step Wave 1 In Manual Control OFF Enable Reverse Operation OFF Step Wave 1 In Velocity (m/s) 300.0 Step Wave 1 In Height 10.0 Step Wave 1 Out Manual Control OFF Step Wave 1 Out Velocity (m/s) 300.0 Step Wave 1 Out Height 1.0 Step Wave 2 Manual Control OFF Step Wave 2 Velocity (m/s) 300.0 Step Wave 2 Height 0.0 Use Manual Step Wave DC ON Step Wave TransferOffset 25.0 Step Wave DiffAperture1 3.0 Step Wave DiffAperture2 0.0 Use Automatic RF Settings FALSE StepWave1RFOffset 100.0 StepWave2RFOffset 300.0 Target Enhancement Enabled FALSE Target Enhancement Mode EDC Target Enhancement Mass 556.0 Target Enhancement Trap Height (V) 12.0 Target Enhancement Extract Height (V) 8.0 Mobility Trapping Manual Release Enabled FALSE Mobility Trapping Release Time (µs) 500 Mobility Trap Height (V) 15.0 Mobility Extract Height (V) 0.0 Trag Gate LUT table enabled FALSE TriWave Trap Gate LookUp Table Using Drift Time Trimming FALSE Drift Time Bins 0 Using Mobility Delay after Trap Release TRUE IMS Wave Delay (µs) 1000 Variable Wave Height Enabled FALSE Wave Height Ramp Type Linear Wave Height Start (V) 10.0 Wave Height End (V) 40.0 Wave Height Using Full IMS TRUE Wave Height Ramp (%) 100.0 Wave Height Look Up Table Variable Wave Velocity Enabled FALSE Wave Velocity Ramp Type Linear Wave Velocity Start (m/s) 1000.0 Wave Velocity End (m/s) 300.0 Wave Velocity Using Full IMS TRUE Wave Velocity Ramp (%) 100.0 Wave Velocity Look Up Table Backing 2.55e0 Source 5.09e-3 Sample Plate 1.00e-6 Trap 1.51e-2 Helium Cell 1.42e3 IMS 3.91e0 Transfer 1.77e-2 TOF 9.08e-7 IMSRFOffset 300 IMSMobilityRFOffset 250 TrapRFOffset 300 Use Automatic RF Settings FALSE AutoStepWave1RFOffset 300 AutoStepWave2RFOffset 350 TransferRFOffset 350 MS Profile Type Auto P MSProfileMass1 100 MSProfileDwellTime1 20 MSProfileRampTime1 20 MSProfileMass2 300 MSProfileDwellTime2 20 MSProfileRampTime2 40 MSProfileMass3 500 PusherInterval 106.000000 PusherOffset 0.250000 LockMassValidSigma 5 Acquisition mass range Start mass 140.000 End mass 800.000 Calibration mass range Start mass 0.000 End mass 0.000 Experiment Reference Compound Name: lockspray_220425 Function Parameters - Function 1 - MOBILITY MS FUNCTION Scan Time (sec) 0.200 Interscan Time (sec) 0.014 Start Mass 140.0 End Mass 800.0 Start Time (mins) 0.00 End Time (mins) 24.00 Data Format Continuum Analyser High Resolution Mode ADC Sample Frequency (GHz) 3.0 ADC Pusher Frequency (µs) 106.0 ADC Pusher Width (µs) 1.50 Use Tune Page Cone Voltage YES Using Auto Trap Collision Energy (eV) 4.000000 Using Auto Transfer Collision Energy (eV) 2.000000 Sensitivity Normal Dynamic Range Normal Save Collapsed Retention Time Data Yes Use Rule File Filtering No FragmentationMode CID Calibration Dynamic 2 Function Parameters - Function 2 - REFERENCE Scan Time (sec) 0.200 Interscan Time (sec) 0.100 Start Mass 140.0 End Mass 800.0 Start Time (mins) 0.00 End Time (mins) 24.00 Data Format Continuum Analyser High Resolution Mode ADC Sample Frequency (GHz) 3.0 ADC Pusher Frequency (µs) 106.0 ADC Pusher Width (µs) 1.50 Use Tune Page Cone Voltage YES Using Auto Trap Collision Energy (eV) 4.000000 Using Auto Transfer Collision Energy (eV) 2.000000 Sensitivity Normal Dynamic Range Normal Save Collapsed Retention Time Data Yes Use Rule File Filtering No FragmentationMode CID [LOCK SPRAY] LockSprayType: Do not apply correction LockSpray Reference Compound Name: lockspray_220425 Scan Time (sec) 0.20 Interval 30 EDC Mass 0.0000 Mass Window +/- 0.5 Scans to Average 3.0 Reference Cone Voltage 40.0 Trap Collision Energy (eV) 4.0 LocksprayDRESetting 99.9000 [METHOD EVENTS:] Timed Events Enabled: YES API Probe Delay Temp: 20.0 Num of Timed Events: 4.0 Event Time Name Action System 1 -60.00 Flow State LC Sample 2 -60.00 Reservoir B LockSpray 3 -60.00 Refill Refill Always LockSpray 4 -60.00 Infusion Start LockSpray Calibration Dynamic 2