Skyline (64-bit) 24.1.0.199 (6a0775ef83) System.IO.IOException: Error occurred running process. Exception in thread "main" java.lang.OutOfMemoryError: GC overhead limit exceeded at gnu.trove.map.hash.TCharObjectHashMap.setUp(TCharObjectHashMap.java:143) at gnu.trove.impl.hash.THash.(THash.java:137) at gnu.trove.impl.hash.THash.(THash.java:104) at gnu.trove.impl.hash.TPrimitiveHash.(TPrimitiveHash.java:68) at gnu.trove.impl.hash.TCharHash.(TCharHash.java:65) at gnu.trove.map.hash.TCharObjectHashMap.(TCharObjectHashMap.java:79) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie$TrieNode.(PeptideTrie.java:72) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie$TrieNode.getOrCreate(PeptideTrie.java:93) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie.(PeptideTrie.java:29) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie.(PeptideTrie.java:15) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideAccessionMatchingTrie.(PeptideAccessionMatchingTrie.java:11) at edu.washington.gs.maccoss.encyclopedia.filereaders.BlibFile.getCopyEntriesToLibrary(BlibFile.java:221) at edu.washington.gs.maccoss.encyclopedia.filereaders.BlibToLibraryConverter.convert(BlibToLibraryConverter.java:141) at edu.washington.gs.maccoss.encyclopedia.cli.ConvertBLIBToLibrary.convert(ConvertBLIBToLibrary.java:64) at edu.washington.gs.maccoss.encyclopedia.cli.ConvertBLIBToLibrary.main(ConvertBLIBToLibrary.java:28) Command-line: C:\Users\giole\AppData\Local\Apps\2.0\74LJTP4N.20P\Z69JC5WL.Y6T\skyl..tion_9286511f3362df93_0018.0001_5aeb0e19d93199bf\Tools\openlogic-openjdk-jre-8u342-b07-windows-64\bin\java.exe -Xmx6098M -cp "C:\Users\giole\AppData\Local\Apps\2.0\74LJTP4N.20P\Z69JC5WL.Y6T\skyl..tion_9286511f3362df93_0018.0001_5aeb0e19d93199bf\Tools\encyclopedia-2.12.30-executable.jar" edu.washington.gs.maccoss.encyclopedia.cli.ConvertBLIBToLibrary -Duser.language=en-US -Duser.region=US -i "C:\Users\giole\Documents\FASTA file\uniprotkb_human_reviewed_plusTrypsin_2024_09_11-z3_nce27-koina-ms2pip_2021_HCD-AlphaPept_rt_generic-EB631BA7683CBEEB296C1CCCD44396C.blib" -f "C:\Users\giole\Documents\FASTA file\uniprotkb_human_reviewed_plusTrypsin_2024_09_11.fasta" -o "C:\Users\giole\Documents\FASTA file\uniprotkb_human_reviewed_plusTrypsin_2024_09_11-z3_nce27-koina.dlib" Working directory: ---> System.IO.IOException: Error occurred running process. Exception in thread "main" java.lang.OutOfMemoryError: GC overhead limit exceeded at gnu.trove.map.hash.TCharObjectHashMap.setUp(TCharObjectHashMap.java:143) at gnu.trove.impl.hash.THash.(THash.java:137) at gnu.trove.impl.hash.THash.(THash.java:104) at gnu.trove.impl.hash.TPrimitiveHash.(TPrimitiveHash.java:68) at gnu.trove.impl.hash.TCharHash.(TCharHash.java:65) at gnu.trove.map.hash.TCharObjectHashMap.(TCharObjectHashMap.java:79) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie$TrieNode.(PeptideTrie.java:72) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie$TrieNode.getOrCreate(PeptideTrie.java:93) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie.(PeptideTrie.java:29) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie.(PeptideTrie.java:15) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideAccessionMatchingTrie.(PeptideAccessionMatchingTrie.java:11) at edu.washington.gs.maccoss.encyclopedia.filereaders.BlibFile.getCopyEntriesToLibrary(BlibFile.java:221) at edu.washington.gs.maccoss.encyclopedia.filereaders.BlibToLibraryConverter.convert(BlibToLibraryConverter.java:141) at edu.washington.gs.maccoss.encyclopedia.cli.ConvertBLIBToLibrary.convert(ConvertBLIBToLibrary.java:64) at edu.washington.gs.maccoss.encyclopedia.cli.ConvertBLIBToLibrary.main(ConvertBLIBToLibrary.java:28) Command-line: C:\Users\giole\AppData\Local\Apps\2.0\74LJTP4N.20P\Z69JC5WL.Y6T\skyl..tion_9286511f3362df93_0018.0001_5aeb0e19d93199bf\Tools\openlogic-openjdk-jre-8u342-b07-windows-64\bin\java.exe -Xmx6098M -cp "C:\Users\giole\AppData\Local\Apps\2.0\74LJTP4N.20P\Z69JC5WL.Y6T\skyl..tion_9286511f3362df93_0018.0001_5aeb0e19d93199bf\Tools\encyclopedia-2.12.30-executable.jar" edu.washington.gs.maccoss.encyclopedia.cli.ConvertBLIBToLibrary -Duser.language=en-US -Duser.region=US -i "C:\Users\giole\Documents\FASTA file\uniprotkb_human_reviewed_plusTrypsin_2024_09_11-z3_nce27-koina-ms2pip_2021_HCD-AlphaPept_rt_generic-EB631BA7683CBEEB296C1CCCD44396C.blib" -f "C:\Users\giole\Documents\FASTA file\uniprotkb_human_reviewed_plusTrypsin_2024_09_11.fasta" -o "C:\Users\giole\Documents\FASTA file\uniprotkb_human_reviewed_plusTrypsin_2024_09_11-z3_nce27-koina.dlib" Working directory: Output: [22:21:30] Indexing uniprotkb_human_reviewed_plusTrypsin_2024_09_11-z3_nce27-koina-ms2pip_2021_HCD-AlphaPept_rt_generic-EB631BA7683CBEEB296C1CCCD44396C.blib ... [22:21:35] Reading BLIB file [22:21:40] Starting alignment 1 with 2478817 data points [22:22:43] Reading Fasta file uniprotkb_human_reviewed_plusTrypsin_2024_09_11.fasta [22:22:43] Reading standard formated FASTA database [22:22:43] Constructing trie from library peptides Exception in thread "main" java.lang.OutOfMemoryError: GC overhead limit exceeded at gnu.trove.map.hash.TCharObjectHashMap.setUp(TCharObjectHashMap.java:143) at gnu.trove.impl.hash.THash.(THash.java:137) at gnu.trove.impl.hash.THash.(THash.java:104) at gnu.trove.impl.hash.TPrimitiveHash.(TPrimitiveHash.java:68) at gnu.trove.impl.hash.TCharHash.(TCharHash.java:65) at gnu.trove.map.hash.TCharObjectHashMap.(TCharObjectHashMap.java:79) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie$TrieNode.(PeptideTrie.java:72) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie$TrieNode.getOrCreate(PeptideTrie.java:93) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie.(PeptideTrie.java:29) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie.(PeptideTrie.java:15) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideAccessionMatchingTrie.(PeptideAccessionMatchingTrie.java:11) at edu.washington.gs.maccoss.encyclopedia.filereaders.BlibFile.getCopyEntriesToLibrary(BlibFile.java:221) at edu.washington.gs.maccoss.encyclopedia.filereaders.BlibToLibraryConverter.convert(BlibToLibraryConverter.java:141) at edu.washington.gs.maccoss.encyclopedia.cli.ConvertBLIBToLibrary.convert(ConvertBLIBToLibrary.java:64) at edu.washington.gs.maccoss.encyclopedia.cli.ConvertBLIBToLibrary.main(ConvertBLIBToLibrary.java:28) ---> System.IO.IOException: Error occurred running process. Exception in thread "main" java.lang.OutOfMemoryError: GC overhead limit exceeded at gnu.trove.map.hash.TCharObjectHashMap.setUp(TCharObjectHashMap.java:143) at gnu.trove.impl.hash.THash.(THash.java:137) at gnu.trove.impl.hash.THash.(THash.java:104) at gnu.trove.impl.hash.TPrimitiveHash.(TPrimitiveHash.java:68) at gnu.trove.impl.hash.TCharHash.(TCharHash.java:65) at gnu.trove.map.hash.TCharObjectHashMap.(TCharObjectHashMap.java:79) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie$TrieNode.(PeptideTrie.java:72) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie$TrieNode.getOrCreate(PeptideTrie.java:93) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie.(PeptideTrie.java:29) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideTrie.(PeptideTrie.java:15) at edu.washington.gs.maccoss.encyclopedia.datastructures.PeptideAccessionMatchingTrie.(PeptideAccessionMatchingTrie.java:11) at edu.washington.gs.maccoss.encyclopedia.filereaders.BlibFile.getCopyEntriesToLibrary(BlibFile.java:221) at edu.washington.gs.maccoss.encyclopedia.filereaders.BlibToLibraryConverter.convert(BlibToLibraryConverter.java:141) at edu.washington.gs.maccoss.encyclopedia.cli.ConvertBLIBToLibrary.convert(ConvertBLIBToLibrary.java:64) at edu.washington.gs.maccoss.encyclopedia.cli.ConvertBLIBToLibrary.main(ConvertBLIBToLibrary.java:28) Command-line: C:\Users\giole\AppData\Local\Apps\2.0\74LJTP4N.20P\Z69JC5WL.Y6T\skyl..tion_9286511f3362df93_0018.0001_5aeb0e19d93199bf\Tools\openlogic-openjdk-jre-8u342-b07-windows-64\bin\java.exe -Xmx6098M -cp "C:\Users\giole\AppData\Local\Apps\2.0\74LJTP4N.20P\Z69JC5WL.Y6T\skyl..tion_9286511f3362df93_0018.0001_5aeb0e19d93199bf\Tools\encyclopedia-2.12.30-executable.jar" edu.washington.gs.maccoss.encyclopedia.cli.ConvertBLIBToLibrary -Duser.language=en-US -Duser.region=US -i "C:\Users\giole\Documents\FASTA file\uniprotkb_human_reviewed_plusTrypsin_2024_09_11-z3_nce27-koina-ms2pip_2021_HCD-AlphaPept_rt_generic-EB631BA7683CBEEB296C1CCCD44396C.blib" -f "C:\Users\giole\Documents\FASTA file\uniprotkb_human_reviewed_plusTrypsin_2024_09_11.fasta" -o "C:\Users\giole\Documents\FASTA file\uniprotkb_human_reviewed_plusTrypsin_2024_09_11-z3_nce27-koina.dlib" Working directory: in pwiz.Common.SystemUtil.ProcessRunner.Run(ProcessStartInfo psi, String stdin, IProgressMonitor progress, IProgressStatus& status, TextWriter writer, ProcessPriorityClass priorityClass, Boolean forceTempfilesCleanup, Func`3 outputAndExitCodeAreGoodFunc, Boolean updateProgressPercentage) in C:\proj\skyline_24_1\pwiz_tools\Shared\CommonUtil\SystemUtil\ProcessRunner.cs:riga 203 --- Fine della traccia dello stack dell'eccezione interna --- --- Fine della traccia dello stack dell'eccezione interna --- in pwiz.Common.SystemUtil.ProcessRunner.ThrowExceptionWithOutput(Exception exception, String output) in C:\proj\skyline_24_1\pwiz_tools\Shared\CommonUtil\SystemUtil\ProcessRunner.cs:riga 263 in pwiz.Common.SystemUtil.ProcessRunner.Run(ProcessStartInfo psi, String stdin, IProgressMonitor progress, IProgressStatus& status, TextWriter writer, ProcessPriorityClass priorityClass, Boolean forceTempfilesCleanup, Func`3 outputAndExitCodeAreGoodFunc, Boolean updateProgressPercentage) in C:\proj\skyline_24_1\pwiz_tools\Shared\CommonUtil\SystemUtil\ProcessRunner.cs:riga 245 in pwiz.Skyline.Model.Lib.EncyclopeDiaHelpers.ConvertKoinaOutputToDlib(String koinaBlibFilepath, String fastaFilepath, String encyclopeDiaDlibFilepath, IProgressMonitor progressMonitor, IProgressStatus& status) in C:\proj\skyline_24_1\pwiz_tools\Skyline\Model\Lib\EncyclopeDiaHelpers.cs:riga 205 in pwiz.Skyline.FileUI.PeptideSearch.EncyclopeDiaSearchControl.Search(EncyclopeDiaSettings settings, CancellationTokenSource token, IProgressStatus status) in C:\proj\skyline_24_1\pwiz_tools\Skyline\FileUI\PeptideSearch\EncyclopeDiaSearchDlg.cs:riga 712