Michael responded: |
2014-08-17 04:06 |
Hi Andre
Thank-you for your note. Skyline does not extract mma for low resolving power instruments like the qtrap. That's why you have a column of NAs. So try just leaving the mma and rp checkboxes blank and see if it runs this time. Also sprocop will only accept a single precursor per peptide. If this is not working I will be happy to take a look at the document myself
Thanks
Michael |
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andre responded: |
2014-08-18 00:04 |
Hi Michael,
thanks for your quick response. However, when starting SProCoP the only checkbox to select is "Using high resolution instrumen" (which I had not selected); also, there is only one precursor per peptide (although in a light and heavy version).
So, it would be great if you could have a look at my document.
Thanks,
Andre |
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Michael responded: |
2014-08-27 10:39 |
Hi Andre -
Still looking into this - Sorry for the delay
Michael |
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Michael responded: |
2014-09-07 09:53 |
Hi Andre -
This is a bug - we will fix it - Thankyou for reporting it - Ill let you know when an updated version is out.
My apologies
Michael |
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brian flatley responded: |
2014-11-28 01:47 |
Hi Michael,
I am having the same problem as Andre, I am trying to monitor 8 peptides across a large number of batches, but I fail to get SProCop to run currently. I have NAs in the average PPM mass error column.
Any help would be great,
Cheers,
Brian |
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Michael responded: |
2014-11-28 06:40 |
Hi Brian -
Thank you for your note. Would you mind sharing the zip file or part of the file? You can send it msberema@ncsu.edu.
Thanks
Michael |
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Michael responded: |
2014-12-03 14:51 |
Hi Brian -
I updated the code so that your file now runs. But I deleted one of your peptides that had several NAs with fwhm. But the fact its SRM data does not matter now. Please let me know if this works for you. Just have to download the new version.
Sorry for the delay
Michael |
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