I have just released Skyline 2.6.0.6851 with fixes for a number of bugs found in the original Skyline 2.6 release. These include:

• Fix to open without error Panorama published and File > Share - Minimized files with newer spectral libraries
• Fix Mascot library building threshold to use homology score instead of identity score (recommended by John Cottrell)
• Fix isotope distributions for 18O labeling
• Fix most common unhandled exception in chromatogram graph
• Fix chromatogram graph full-scan tracking dot not showing up with Split Graph on MS1-only data
• Fix unhandled exception in Peptide Settings validation of spectral library IMS resolving power
• Fix changing DIA isolation scheme not updating peptides with all precursors removed
• Fix Import Peptide Search wizard removing to much when remove prefix option is chosen
• Catch errors in Start page and Tool Store when no internet connection
• Better tie-breaking for q values in MSF files

Skyline should ask you to update automatically, after you restart twice. If it does not, please follow the advice on this page before posting to the support board for help updating:

https://skyline.gs.washington.edu/labkey/wiki/home/software/Skyline/page.view?name=tip_recover_install

Thank you for using Skyline in your research.

Brendan MacLean Skyline Project Lead