Scientist, Analytical Chemistry - Protein Analytics, Emeryville, CA, USA

Scientist, Analytical Chemistry - Protein Analytics, Emeryville, CA, USA ana  2021-05-18

Zymergen has an opportunity for a scientist who will be working on protein identification and quantitation using liquid chromatography coupled with high resolution, accurate mass tandem mass spectrometry, triple quadrupole mass spectrometry, and UV detection.

Job Description and Specific Responsibilities

Immediate responsibilities include:

Prepare samples from small and large scale microbial fermentations.
Perform multi-step sample preparations including enzymatic digestion (solution and in-gel), reduction/alkylation, buffer exchange, deglycosylation, sample concentration, etc.
Optimization and development of new sample preparation methods including high throughput sample preparation.
Method development and optimization for transfer to non-mass spectrometry, LC-UV-based techniques.
Operation and maintenance of LC-MS equipment including performance of system suitability tests.
Perform data analysis.
Evaluate new technologies and platforms.
Write and review formal reports, including method SOPs and other technical documents and present findings in technical team meetings and to collaborators.
Lead, mentor, and inspire junior team members.


The favor candidates with the following profile:

Hands-on experience in protein analytics and characterization (top-down, bottom-up, and targeted proteomics) by LC/MS, including intact mass determination, glycosylation and analysis of other PTMs.
Expertise in sample preparation techniques for protein analysis such as enzymatic hydrolysis including in-gel digestion.
Knowledge of chromatography techniques used for protein characterization (RP, size exclusion).
Proficiency in LC and MS-related software, such as Agilent MassHunter, Skyline, Agilent BioConfirm, Thermo Xcalibur, PEAKS.
Experience with liquid handling and automation systems is a plus.
Experience with small molecule determination and quantification is a plus.
Proven track record of initiative, attention to detail, collaborating and communicating within a team.

link to apply: