wildsmith.kristin responded: |
2014-04-21 10:44 |
for 2. we found a link in a separate thread and installed Quasar 1.2. ( http://www.broadinstitute.org/~manidr/QuaSAR-1_2.zip. )We now see the "standard present" box, and the response curves were successfully generated.
I encountered the "required columns" are missing error again, unfortunately. |
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minliecn responded: |
2015-07-12 20:28 |
I am practicing the QuaSAR tutorial with Skyline V3.1 and QuaSAR1_32. I have encountered the same errors as listed in the third ppt slide by Kristin. Does anyone has a solution for the issue? Thanks! |
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Mani responded: |
2015-07-13 06:08 |
I think this error is caused by an incorrect default for the "Number of transitions to plot" in the "Plots" tab. If you manually select a number of transitions to display (3 is a good default), this error should go away. I'll soon release a new version with this problem fixed. |
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minliecn responded: |
2015-07-13 08:53 |
Thanks for your help, Mani. I have a different question now. when I am trying to play with my own dataset, skyline was complaining about "Error in calculate(data.subset, concentrationFile, output.prefix = outputPrefix, :
No data for LOD calculation -- disable LOD calculation". Do you know which part is causing this issue? Thanks very much for your help. |
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Mani responded: |
2015-07-13 12:02 |
As the error message points out, QuaSAR is not able to calculate LODs using your data. This could be because there are no blank samples (with concentration = 0), or not enough replicates to calculate standard deviations. If you uncheck the "LOD/LOQ table" checkbox, the error should go away. Obviously, you won't get LOD/LOQ tables, but calibration curves and CV values will be output. If the error persists, may be you can send me the data (as an export, or as a Skyline file) so I can try to debug the problem. |
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