question about Skyline deal with small molecular SRM longzhen8866  2024-09-05 02:04
 

There are many transitions listed for one precursor. I just want to keep the highest 3 transitions when I export to be a SRM method. How can I do that?

 
 
Brian Pratt responded:  2024-09-05 08:20

Have a look at this tutorial:

https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_method_refine

It is presented in terms of peptides but the refinement principles are the same for small molecules.

Thanks for using the Skyline support board,

Brian Pratt