MS1 filtering by DDA on FAIMS-Lumos: Rugged Extracted ion chromatograms | alejandro.cohen | 2022-08-16 12:28 | |||||||||||||||||||||||||||||||||||||||||||||
Long story short: We recently got a Lumos with Faims source, and I run our BSA samples we run as QCs using CV of 40, 60 and 80 in DDA mode. I created a skyline document using the Import DDA Peptide Search, using the .raw files and CRAP Fasta file (Contains BSA) and Fragger (I tried Amanda too). I get the expected proteins and peptides, but the XICs appear rugged, which I suspect corresponds to the traces for the different CVs. Cheers Alejandro |
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