Multiplex Orbitrap Data - Cannot get Skyline to Recognize SIL Peptide Transitions | lamarj | 2022-02-18 11:27 | |||||||||
To whom it may concern, We are trying to process data acquired with an Orbitrap Fusion Lumos. It looks like skyline has the correct predictions for transitions (we observe several in the .raw file, please see attached screenshot) but they are not being recognized/plotted in the chromatogram. I have tried just about everything I can think of in the transition settings. Skyline does "identify" non-labeled transitions, but transitions associated with the SIL peptides are not identified. Currently using version 21.2.0.369 Thanks, Jason |
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