Hi Skyline,
Happy New Year!
I am viewing some results and I keep getting #N/A values in for Normalised Area in the Calibration curve grid.
I don't understand why? as Skyline can clearly see all 3 transitions (As shown in the image attached) but I keep getting that error?
The image shows 3 replicates of a peptide called : "GWVTDGFSSLK" and you can see all 3 transitions of the peptide detected at each replicate but as highlighted in blue, replicates 1 (31L_1) and 2 (31L_2) are showing #N/A values.
Is there a way I can fix this or is there something I am doing wrong?
Thanks,
Shimon