skyline is not able identify with my iRT peptides | Sangram | 2021-12-07 05:32 | |||||||||||||||
Hello people, I am trying to perform a (pretty straightforward) DIA analysis with 32 fractions of a cell line proteome. I performed the spectra search using PeptideShaker and yielded the results in mzid format. Now in the "Add iRT peptide" window all samples shows failed. I would like to know what implications I might have down the analysis. This is because I noticed whenever this happens and I would export a spectral library in openSWATH format, the values from RetentionTimeCalibrationScore is missing ! How can I stop this from happening ? |
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