Failed importing DIA-PASEF results and isolation schemes | msielaff | 2021-09-02 07:28 | |||||||||||||||||||||||||||||||||||||||||||||||||||
Dear Skyline team, I'm trying to set up a Skyline document for a AutoQC-Panorama pipeline in order to monitor some peptides as QC from HeLa DIA-PASEF runs. Unfortunately, Skyline fails to import the results from some of our DIA-PASEF methods with following error: At 3:23 PM: Failed importing results file 'O:\RAW_ttp\O210827_003_Slot1-1_1_3239.d'. error reading spectrum merged=12 frame=5 scanStart=735 scanEnd=937 pwiz.Skyline.Model.Results.ChromCacheBuildException: Failed importing results file 'O:\RAW_ttp\O210827_003_Slot1-1_1_3239.d'. error reading spectrum merged=12 frame=5 scanStart=735 scanEnd=937 ---> System.Reflection.TargetInvocationException: error reading spectrum merged=12 frame=5 scanStart=735 scanEnd=937 ---> System.Exception: error reading spectrum merged=12 frame=5 scanStart=735 scanEnd=937 ---> System.Exception: Scan number [936] out of range. at pwiz.CLI.msdata.SpectrumList.spectrum(Int32 index, DetailLevel detailLevel) at pwiz.ProteowizardWrapper.MsDataFileImpl.GetCachedSpectrum(Int32 scanIndex, DetailLevel detailLevel) in C:\proj\skyline_21_1_x64\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 1155 at pwiz.ProteowizardWrapper.MsDataFileImpl.GetSpectrum(Int32 spectrumIndex) in C:\proj\skyline_21_1_x64\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 893 at pwiz.Skyline.Model.Results.SpectraChromDataProvider.Spectra.ReadSpectrum(Int32& i) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 1075 --- End of inner exception stack trace --- at pwiz.Skyline.Model.Results.SpectraChromDataProvider.Spectra.ReadSpectrum(Int32& i) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 1126 at pwiz.Skyline.Model.Results.SpectraChromDataProvider.Spectra.Read() in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 953 at pwiz.Skyline.Model.Results.SpectraChromDataProvider.Spectra.<RunAsync>b__33_0() in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 810 --- End of inner exception stack trace --- at pwiz.Skyline.Util.Helpers.WrapAndThrowException(Exception x) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Util\Util.cs:line 1940 at pwiz.Skyline.Model.Results.SpectraChromDataProvider.Spectra.NextSpectrum() in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 916 at pwiz.Skyline.Model.Results.SpectraChromDataProvider.ExtractChromatogramsLocked() in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 259 at pwiz.Skyline.Model.Results.SpectraChromDataProvider.ExtractChromatograms() in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 239 at pwiz.Skyline.Model.Results.SpectraChromDataProvider.SetRequestOrder(IList`1 chromatogramRequestOrder) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 592 at pwiz.Skyline.Model.Results.ChromCacheBuilder.Read(ChromDataProvider provider) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 384 at pwiz.Skyline.Model.Results.ChromCacheBuilder.BuildCache() in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 252 --- End of inner exception stack trace --- Skyline also fails when I try to import the isolation scheme from the raw file: --------------------------- Skyline --------------------------- Failed reading isolation scheme. Scan number [936] out of range. --------------------------- OK More Info --------------------------- System.Reflection.TargetInvocationException: Scan number [936] out of range. ---> System.Exception: Scan number [936] out of range. at pwiz.CLI.msdata.SpectrumList.spectrum(Int32 index, DetailLevel detailLevel) at pwiz.ProteowizardWrapper.MsDataFileImpl.GetCachedSpectrum(Int32 scanIndex, DetailLevel detailLevel) in C:\proj\skyline_21_1_x64\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 1155 at pwiz.ProteowizardWrapper.MsDataFileImpl.GetSpectrum(Int32 spectrumIndex) in C:\proj\skyline_21_1_x64\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 893 at pwiz.Skyline.Model.IsolationSchemeReader.ReadIsolationRanges(MsDataFileUri dataSource, IsolationRange[] isolationRanges) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\IsolationSchemeReader.cs:line 159 at pwiz.Skyline.Model.IsolationSchemeReader.ReadIsolationRangesFromFiles(IProgressMonitor progressMonitor) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\IsolationSchemeReader.cs:line 62 at pwiz.Skyline.Model.IsolationSchemeReader.Import(String name, IProgressMonitor progressMonitor) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\IsolationSchemeReader.cs:line 44 at pwiz.Skyline.SettingsUI.EditIsolationSchemeDlg.<>c__DisplayClass74_1.<ImportRangesFromFiles>b__1(IProgressMonitor progressMonitor) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\SettingsUI\EditIsolationSchemeDlg.cs:line 1045 at pwiz.Skyline.Controls.LongWaitDlg.RunWork(Action`1 performWork) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 254 --- End of inner exception stack trace --- at pwiz.Skyline.Util.Helpers.WrapAndThrowException(Exception x) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Util\Util.cs:line 1940 at pwiz.Skyline.Controls.LongWaitDlg.PerformWork(Control parent, Int32 delayMillis, Action`1 performWork) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 202 at pwiz.Skyline.Controls.LongWaitDlg.PerformWork(Control parent, Int32 delayMillis, Action`1 performWork) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 140 at pwiz.Skyline.SettingsUI.EditIsolationSchemeDlg.ImportRangesFromFiles(MsDataFileUri[] dataSources) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\SettingsUI\EditIsolationSchemeDlg.cs:line 1045 --------------------------- Based on these error messages, do you have any idea what the problem could be and how to solve it? Many thanks, Malte |
|||||||||||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||||||||||