Different dotp value in peak area view and target window
meharunnissakohli
2021-08-13 00:15
Dear Support Team,
I was using Skyline for analyzing an MRM based data and also using a spectral library. During analysis, for some of the peptides, I noticed that the dotp value shown in 'peak area view' and that in 'target window' are different. However, for most of the other peptides, it is same at both the places.
Could you please help me understand the problem. Looking forward to hearing from you.
Attached here is the screenshot for the same.
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Brendan MacLean responded:
2021-08-13 15:16
Hi Mehar,
Can you try turning on Settings > Integrate All? When I look at your peak areas plot it looks to me like a fair number of transitions are not represented in the empirical data. I am still a little surprised that the targets view and the peak areas plot aren't showing the same dotp, but it may be related to not having "Integrate All" checked.
peak area.JPG
target window.JPG