Hi,
I did a targeted analysis in Skyline using a library build using Prosit as a data source (Library --> Build --> Prosit). All worked out great, but as a final quality control and as a figure in a presentation, I want to compare MS/MS spectra from my runs against the library spectra, following the old View --> MS/MS spectra option. However, when I use View --> Library Match I can only generate plots between Prosit and my library (conveniently, I get a perfect dotp score as my Prosit-build library spectrum is compared with, well, Prosit).
Nevertheless, such a mirror plot with a measured MS/MS spectrum against the library would be great, although I would also settle for the old style without the mirror option, but it seems to no longer exist or I am just unable to find it. Search function sadly does not help as the old View --> MS/MS spectrum document is still online under "Tips", so maybe someone could point me in the right direction...?
Thank you in advance,
Jan