Request: Visualize reporter ions in "Library Match" window Juan C. Rojas E.  2021-06-11 05:44
 

Hi,

I was wondering if it is possible to visualize modification specific reporter ions in the library match or if it is a feature that could be implemented.

I have managed to show some modification specific neutral losses (Slide 1; e.g. y7-331), but there are several other ions that I can annotate to derivatization tag specific fragments that I regularly check and would be really nice if this were also highlighted for my validation steps and consideration for fragment transitions to track.

As always, thanks for the awesome work and support.
Sincerely,
JC
 
 
Nick Shulman responded:  2021-06-11 09:39
It appears that this feature was requested a septennium ago, but we have not gotten around to implementing the fix:
https://skyline.ms/issues/home/issues/details.view?issueId=352

If you could send us a small Skyline document that illustrates this problem, I will attach the .sky.zip file to the open Issue. Having an example attached there will probably make it easier for the person who eventually fixes this bug.
--Nick
 
Juan C. Rojas E. responded:  2021-06-14 00:23
Hi Nick,

Attached is a small Skyline document for peptides with the modification-specific fragments described in the Excel Sheet (attached as well) for "ARP_lys_ox" and "ARP_arg_ox".

Let me know if I can help in any other way.

Thank your for the time and support.
Sincerely,
Juan C.